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Literature summary extracted from

  • El-Sersy, N.; Abd-Elnaby, H.; Abou-Elela, G.; Ibrahim, H.; El-Toukhy, N.
    Optimization, economization and characterization of cellulase produced by marine Streptomyces ruber (2010), Afr. J. Biotechnol., 9, 6355-6364.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 24000
-
 x * 27000, tpe I CMCase, SDS-PAGE, x * 24000, type II CMCase, SDS-PAGE Streptomyces ruber
3.2.1.4 27000
-
 x * 27000, tpe I CMCase, SDS-PAGE, x * 24000, type II CMCase, SDS-PAGE Streptomyces ruber

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Streptomyces ruber
-
 contains two different types of CMCases type I and II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 isolation and separation of two different types of CMCases, type I and II, by anion exchange chromatography Streptomyces ruber

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.4 additional information optimization of culture conditions for production of carboxymethyl cellulase using Plackett-Burman design. Optimized conditions include high concentration of KH2PO4, low concentration of MgSO4, and rice straw at 30 g/l as low cost source of cellulose Streptomyces ruber
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 846.8
-
 purified type II CMCase, pH 4.8, 40°C Streptomyces ruber
3.2.1.4 4240
-
 purified type I CMCase, pH 4.8, 40°C Streptomyces ruber

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O
-
 Streptomyces ruber ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 27000, tpe I CMCase, SDS-PAGE, x * 24000, type II CMCase, SDS-PAGE Streptomyces ruber

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 Carboxymethyl cellulase
-
 Streptomyces ruber
3.2.1.4 CMCase
-
 Streptomyces ruber

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 40
-
-
 Streptomyces ruber

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 30 60 activity range Streptomyces ruber

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6
-
-
 Streptomyces ruber

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 5 9 activity range Streptomyces ruber