EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.3 | industry | this glucoamylase may find important applications in the starch saccharification industry and in bioethanol production | Aureobasidium pullulans |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.3 | gene APGA1, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris | Aureobasidium pullulans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.3 | extracellular | - |
Aureobasidium pullulans | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 66000 | - |
x * 66000, SDS-PAGE | Aureobasidium pullulans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.3 | Aureobasidium pullulans | E2GDF4 | gene APGA1 | - |
3.2.1.3 | Aureobasidium pullulans NRRL 12974 | E2GDF4 | gene APGA1 | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.3 | glycoprotein | - |
Aureobasidium pullulans |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.3 | recombinant enzyme from Pichia pastoris by ultrafiltration, gel filtration, and anion exchange chromatography to homogeneity | Aureobasidium pullulans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 298 | - |
purified recombinant enzyme, pH 4.5, 60°C, substarte raw potato starch | Aureobasidium pullulans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.3 | amylopectin + H2O | - |
Aureobasidium pullulans | ? | - |
? | |
3.2.1.3 | amylopectin + H2O | - |
Aureobasidium pullulans NRRL 12974 | ? | - |
? | |
3.2.1.3 | amylose + H2O | - |
Aureobasidium pullulans | ? | - |
? | |
3.2.1.3 | amylose + H2O | - |
Aureobasidium pullulans NRRL 12974 | ? | - |
? | |
3.2.1.3 | glycogen + H2O | - |
Aureobasidium pullulans | ? | - |
? | |
3.2.1.3 | glycogen + H2O | - |
Aureobasidium pullulans NRRL 12974 | ? | - |
? | |
3.2.1.3 | additional information | poor activity with pullulan and laminarin | Aureobasidium pullulans | ? | - |
? | |
3.2.1.3 | additional information | poor activity with pullulan and laminarin | Aureobasidium pullulans NRRL 12974 | ? | - |
? | |
3.2.1.3 | raw potato starch + H2O | - |
Aureobasidium pullulans | ? | - |
? | |
3.2.1.3 | soluble starch + H2O | - |
Aureobasidium pullulans | ? | - |
? | |
3.2.1.3 | soluble starch + H2O | - |
Aureobasidium pullulans NRRL 12974 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.3 | ? | x * 66000, SDS-PAGE | Aureobasidium pullulans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.3 | APGA1 | - |
Aureobasidium pullulans |
3.2.1.3 | glucoamylase | - |
Aureobasidium pullulans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 60 | - |
- |
Aureobasidium pullulans |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 30 | 80 | activity range | Aureobasidium pullulans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 4.5 | - |
- |
Aureobasidium pullulans |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 3 | 6 | purified recombinant enzyme, stable between pH 3 and pH 6. The catalytic activity of the enzyme is reduced quickly at higher than pH 5 and is totally lost at higher than pH 7 | Aureobasidium pullulans |