Literature summary extracted from

Zhang, Y.; Edwards, T.E.; Begley, D.W.; Abramov, A.; Thompkins, K.B.; Ferrell, M.; Guo, W.J.; Phan, I.; Olsen, C.; Napuli, A.; Sankaran, B.; Stacy, R.; Van Voorhis, W.C.; Stewart, L.J.; Myler, P.J.
Structure of nitrilotriacetate monooxygenase component B from Mycobacterium thermoresistibile (2011), Acta Crystallogr. Sect. F, 67, 1100-1105.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.14.14.10	expressed in Escherichia coli BL21(DE3) Rosetta cells	Mycolicibacterium thermoresistibile

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.14.14.10	sitting drop vapor diffusion method, using 0.2 M magnesium chloride, 0.1 M MES pH 6.0 and 20% PEG 6000	Mycolicibacterium thermoresistibile

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.14.10	Mycolicibacterium thermoresistibile	E5Q9D7	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.14.14.10	HisTrap FF column chromatography and Superdex 75 gel filtration	Mycolicibacterium thermoresistibile

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.14.10	nitrilotriacetate + FMNH2 + H+ + O2	-	Mycolicibacterium thermoresistibile	iminodiacetate + glyoxylate + FMN + H2O	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.14.14.10	homodimer	x-ray crystallography	Mycolicibacterium thermoresistibile

Synonyms

EC Number	Synonyms	Comment	Organism
1.14.14.10	nitrilotriacetate monooxygenase component B	-	Mycolicibacterium thermoresistibile
1.14.14.10	NTA-MoB	-	Mycolicibacterium thermoresistibile

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.14.14.10	FMNH2	-	Mycolicibacterium thermoresistibile

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Release 2023.1 (January 2023)