Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Grishin, A.; Ajamian, E.; Zhang, L.; Cygler, M.
    Crystallization and preliminary X-ray analysis of PaaAC, the main component of the hydroxylase of the Escherichia coli phenylacetyl-coenzyme A oxygenase complex (2010), Acta Crystallogr. Sect. F, 66, 1045-1049.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.149 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.149 hanging drop vapor diffusion method, in the absence of a bound ligand (with 100 mM N-(2-acetamido)-iminodiacetic acid pH 5.5) as well as in complexes with CoA, 3-hydroxybutyryl-CoA, benzoyl-CoA and phenylacetyl-CoA, using either 0.1 M PIPES pH 6.5, 15% (w/v) PEG 550 monomethyl ether or 0.1 M PIPES pH 6.5, 5% (v/v) 2-propanol, 5% (w/v) PEG 550 monomethyl ether Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.149 additional information the enzyme shows low affinity for iron, some rearrangement of the protein is induced by iron binding Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.149 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.149 Ni-NTA column chromatography and Superose 12 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.149 phenylacetyl-CoA + O2 + NADPH + H+ true substrate Escherichia coli 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA + H2O + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.149 PaaAC
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.149 NADPH
-
 Escherichia coli