Literature summary extracted from

Lack, N.; Lowe, E.; Liu, J.; Eltis, L.; Noble, M.; Sim, E.; Westwood, I.
Structure of HsaD, a steroid-degrading hydrolase, from Mycobacterium tuberculosis (2007), Acta Crystallogr. Sect. F, 64, 2-7.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.7.1.17	expressed in Pseudomonas putida strain KT4224	Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.7.1.17	sitting drop vapor diffusion method, using 30% (w/v) PEG 3000, 0.1?M CHES, pH 9.5	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3.7.1.17	4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid + H2O	Mycobacterium tuberculosis	high specificity	?	-	?
3.7.1.17	4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid + H2O	Mycobacterium tuberculosis H37Rv	high specificity	?	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.7.1.17	Mycobacterium tuberculosis	-	-	-
3.7.1.17	Mycobacterium tuberculosis H37Rv	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.7.1.17	Ni affinity column chromatography	Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.7.1.17	4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid + H2O	high specificity	Mycobacterium tuberculosis	?	-	?
3.7.1.17	4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid + H2O	high specificity	Mycobacterium tuberculosis H37Rv	?	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.7.1.17	tetramer	x-ray crystallography	Mycobacterium tuberculosis

Synonyms

EC Number	Synonyms	Comment	Organism
3.7.1.17	HsaD	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)