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Literature summary extracted from

  • Spier, M.; Fendrich, R.; Almeida, P.; Noseda, M.; Greiner, R.; Konietzny, U.; Woiciechowski, A.; Soccol, V.; Soccol, C.
    Phytase produced on citric byproducts: purification and characterization (2011), World J. Microbiol. Biotechnol., 27, 267-274.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 additional information
-
 additional information typical Michaelis-Menten kinetics Aspergillus niger
3.1.3.8 0.52
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate pH 5.5, 50°C Aspergillus niger
3.1.3.8 0.93
-
 4-nitrophenyl phosphate pH 5.5, 50°C Aspergillus niger
3.1.3.8 1.75
-
 ATP pH 5.5, 50°C Aspergillus niger
3.1.3.8 2.5
-
 ADP pH 5.5, 50°C Aspergillus niger
3.1.3.8 3.8
-
 1-naphthyl phosphate pH 5.5, 50°C Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.8 extracellular
-
 Aspergillus niger
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 108000
-
 x * 108000, SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Aspergillus niger
-
 ? + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Aspergillus niger
-
 isolated from soil samples collected in Northeast Parana State in Brazil
-
3.1.3.8 Aspergillus niger FS3
-
 isolated from soil samples collected in Northeast Parana State in Brazil
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 native enzyme 10.1fold by cationic exchange chromatography, anionic exchange chromatography, and chromatofocusing Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.8 additional information growth on citric pulp fermentation Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 86.1
-
 purified native enzyme, pH 5.5, 50°C, substrate phytate Aspergillus niger

Storage Stability

EC Number Storage Stability Organism
3.1.3.8 4°C, purified native enzyme, after 30 days, 88.9% activity are remaining, after 60 days the remaining activity is 84.25% of initial activity, and after 180 days 57.5% Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 1-naphthyl phosphate + H2O
-
 Aspergillus niger 1-naphthol + phosphate
-
 ?
3.1.3.8 1-naphthyl phosphate + H2O
-
 Aspergillus niger FS3 1-naphthol + phosphate
-
 ?
3.1.3.8 4-nitrophenyl phosphate + H2O
-
 Aspergillus niger 4-nitrophenol + phosphate
-
 ?
3.1.3.8 4-nitrophenyl phosphate + H2O
-
 Aspergillus niger FS3 4-nitrophenol + phosphate
-
 ?
3.1.3.8 ADP + H2O
-
 Aspergillus niger AMP + phosphate
-
 ?
3.1.3.8 ADP + H2O
-
 Aspergillus niger FS3 AMP + phosphate
-
 ?
3.1.3.8 ATP + H2O
-
 Aspergillus niger ADP + phosphate
-
 ?
3.1.3.8 ATP + H2O
-
 Aspergillus niger FS3 ADP + phosphate
-
 ?
3.1.3.8 additional information substrate specificity with substrates Na-phytate, i.e. myo-inositol-1,2,3,4,5,6-hexakisphosphate, naphtyl-1 phosphate, 4-nitrophenylphosphate, ATP, and ADP, overview Aspergillus niger ?
-
 ?
3.1.3.8 additional information substrate specificity with substrates Na-phytate, i.e. myo-inositol-1,2,3,4,5,6-hexakisphosphate, naphtyl-1 phosphate, 4-nitrophenylphosphate, ATP, and ADP, overview Aspergillus niger FS3 ?
-
 ?
3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
 Aspergillus niger ? + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 ? x * 108000, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 acid phytase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 60
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 60
-
 purified enzyme, residual activity of 86.0%, 68.4%, and 48.2% after 10, 20 or 40 min, respectively Aspergillus niger
3.1.3.8 70
-
 purified enzyme, 40 min Aspergillus niger
3.1.3.8 80
-
 purified enzyme, 30 min, 30.5% activity remaining Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5.5
-
-
 Aspergillus niger