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Literature summary extracted from

  • Tian, Y.; Peng, R.; Xu, J.; Zhao, W.; Gao, F.; Fu, X.; Xiong, A.; Yao, Q.
    Mutations in two amino acids in phyI1s from Aspergillus niger 113 improve its phytase activity (2010), World J. Microbiol. Biotechnol., 26, 903-907.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.3.8 analysis gain of function mutants of the enzyme can be instrumental for the structure-function study of the enzyme and for industrial application Aspergillus niger

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 gene phyI1s, DNA and amino acid sequence determination and analysis, expression of N-terminally His6-tagged wild-type and mutant enzymes Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.8 E121F random mutagenesis, the mutant shows 3.1fold increased activity and 3.24fold reduced affinity for sodium phytate compared to the wild-type enzyme Aspergillus niger
3.1.3.8 K41E random mutagenesis, the mutant shows 2.5fold increased activity and 1.78fold reduced affinity for sodium phytate compared to the wild-type enzyme Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 1.98
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate purified recombinant His-tagged mutant E121F, pH 5.0, 37°C Aspergillus niger
3.1.3.8 3.024
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate purified recombinant His-tagged mutant K41E, pH 5.0, 37°C Aspergillus niger
3.1.3.8 8.442
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate purified recombinant His-tagged wild-type enzyme, pH 5.0, 37°C Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Aspergillus niger
-
 ? + phosphate
-
 ?
3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Aspergillus niger 113
-
 ? + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Aspergillus niger
-
 gene phyI1s
-
3.1.3.8 Aspergillus niger 113
-
 gene phyI1s
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 recombinant N-terminally His6-tagged wild-type and mutant enzymes by nickel affinity chromatography Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 8.82
-
 purified recombinant His-tagged wild-type enzyme, pH 5.0, 37°C Aspergillus niger
3.1.3.8 22.1
-
 purified recombinant His-tagged mutant K41E, pH 5.0, 37°C Aspergillus niger
3.1.3.8 27.28
-
 purified recombinant His-tagged mutant E121F, pH 5.0, 37°C Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
 Aspergillus niger ? + phosphate
-
 ?
3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
 Aspergillus niger 113 ? + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 More structural model of the wild type and mutant enzymes, overview Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 60
-
-
 Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.8 59.83
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate purified recombinant His-tagged mutant E121F, pH 5.0, 37°C Aspergillus niger
3.1.3.8 83.67
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate purified recombinant His-tagged mutant K41E, pH 5.0, 37°C Aspergillus niger
3.1.3.8 161
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate purified recombinant His-tagged wild-type enzyme, pH 5.0, 37°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 2
-
 optimum 1 Aspergillus niger
3.1.3.8 5
-
 optimum 2 Aspergillus niger

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.8 19.17
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate purified recombinant His-tagged wild-type enzyme, pH 5.0, 37°C Aspergillus niger
3.1.3.8 27.83
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate purified recombinant His-tagged mutant K41E, pH 5.0, 37°C Aspergillus niger
3.1.3.8 30.5
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate purified recombinant His-tagged mutant E121F, pH 5.0, 37°C Aspergillus niger