EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.280 | expression in Escherichia coli | Vibrio proteolyticus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.4.1.280 | crystals obtained at 4°C using the hanging-drop vapor interactions diffusion method by mixing 5 microl of a protein solution with 5 microl of a reservoir solution comprising 0.1 M HEPES Na-OH (pH 8.0), 0.17 M CaCl2, and 30%-40% (v/v) PEG400 | Vibrio proteolyticus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.280 | cytosol | - |
Vibrio proteolyticus | 5829 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.280 | N,N'-diacetylchitobiose + phosphate | Vibrio proteolyticus | - |
N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
2.4.1.280 | N,N'-diacetylchitobiose + phosphate | Vibrio proteolyticus | the enzymatic phosphorolysis of ChBP begins with the direct nucleophilic attack to the glycosidic bond with the aid of D492, which donates a proton to the glycosidic oxygen atom, and then proceeds through an oxocarbenium cation activated by ion-like transition state | N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.280 | Vibrio proteolyticus | Q76IQ9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.280 | - |
Vibrio proteolyticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.280 | N,N'-diacetylchitobiose + phosphate | - |
Vibrio proteolyticus | N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
2.4.1.280 | N,N'-diacetylchitobiose + phosphate | the enzymatic phosphorolysis of ChBP begins with the direct nucleophilic attack to the glycosidic bond with the aid of D492, which donates a proton to the glycosidic oxygen atom, and then proceeds through an oxocarbenium cation activated by ion-like transition state | Vibrio proteolyticus | N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.280 | chbP | - |
Vibrio proteolyticus |
2.4.1.280 | chitobiose phosphorylase | - |
Vibrio proteolyticus |