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Literature summary extracted from

  • Pai, C.H.; Wu, H.J.; Lin, C.H.; Wang, A.H.
    Structure and mechanism of Escherichia coli glutathionylspermidine amidase belonging to the family of cysteine; histidine-dependent amidohydrolases/peptidases (2011), Protein Sci., 20, 557-566.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.78 mutant enzyme C59A is expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.78 mutant enzyme C59A in complex with glutathionylspermidine Escherichia coli
6.3.1.8 mutant C59A lacking amidase activity, in complex with amidase substrate gluthionylspermidine and ADP. Homodimer, and each monomer contains the N-terminal amidase and C-terminal synthetase domains connected by a linker in between Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.78 C59A active site mutant Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.78 glutathionylspermidine + H2O Escherichia coli
-
 glutathione + spermidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.78 Escherichia coli P0AES0 bifunctional enzyme glutathionylspermidine synthetase/amidase
-
6.3.1.8 Escherichia coli P0AES0 bifunctional glutathionylspermidine synthetase/amidase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.78 nickel affinity column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.78 glutathionylspermidine + H2O
-
 Escherichia coli glutathione + spermidine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.78 Glutathionylspermidine synthetase/amidase bifunctional enzyme Escherichia coli
3.5.1.78 GSP amidase
-
 Escherichia coli
3.5.1.78 GspA glutathionylspermidine amidase domain of GspSA Escherichia coli
3.5.1.78 GspSA bifunctional enzyme Escherichia coli