Literature summary extracted from
Nakazawa, Y.; Sagane, Y.; Kikuchi, T.; Uchino, M.; Nagai, T.; Sato, H.; Toeda, K.; Takano, K.
Purification, biochemical characterization, and cloning of phospholipase D from Streptomyces racemochromogenes strain 10-3 (2010), Protein J., 29, 598-608.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.4.4 |
gene pld103, DNA and amino acid sequence determination and analysis, sequence comparisons |
Streptomyces racemochromogenes |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.1.4.4 |
Ca2+ |
required |
Streptomyces racemochromogenes |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.1.4.4 |
55000 |
- |
x * 55000, SDS-PAGE |
Streptomyces racemochromogenes |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.4.4 |
Streptomyces racemochromogenes |
E0D7I2 |
- |
- |
3.1.4.4 |
Streptomyces racemochromogenes 10-3 |
E0D7I2 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.4.4 |
native enzyme 17.4fold by ammonium sulfate fractionation, and cation and anion exchange chromatography |
Streptomyces racemochromogenes |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.1.4.4 |
374.3 |
- |
purified PLD103, pH 7.5, 37°C |
Streptomyces racemochromogenes |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.4.4 |
phosphatidylcholine + H2O |
egg yolk lecithin |
Streptomyces racemochromogenes |
choline + phosphatidate |
- |
? |
|
3.1.4.4 |
phosphatidylcholine + H2O |
egg yolk lecithin |
Streptomyces racemochromogenes 10-3 |
choline + phosphatidate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.1.4.4 |
? |
x * 55000, SDS-PAGE |
Streptomyces racemochromogenes |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.4.4 |
PLD103 |
- |
Streptomyces racemochromogenes |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.1.4.4 |
50 |
- |
- |
Streptomyces racemochromogenes |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.1.4.4 |
10 |
60 |
over 80% of the activity remaining after 10 min within the temperature range |
Streptomyces racemochromogenes |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.1.4.4 |
7.5 |
- |
PLD103 exerts the highest hydrolytic activity at a slightly alkaline pH |
Streptomyces racemochromogenes |
pH Stability
EC Number |
pH Stability |
pH Stability Maximum |
Comment |
Organism |
---|
3.1.4.4 |
4 |
9 |
purified enzyme, over 70% activity remaining after 4 h |
Streptomyces racemochromogenes |
pI Value
EC Number |
Organism |
Comment |
pI Value Maximum |
pI Value |
---|
3.1.4.4 |
Streptomyces racemochromogenes |
isoelectric focusing |
- |
8 |