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Literature summary extracted from

  • Nakazawa, Y.; Sagane, Y.; Kikuchi, T.; Uchino, M.; Nagai, T.; Sato, H.; Toeda, K.; Takano, K.
    Purification, biochemical characterization, and cloning of phospholipase D from Streptomyces racemochromogenes strain 10-3 (2010), Protein J., 29, 598-608.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.4 gene pld103, DNA and amino acid sequence determination and analysis, sequence comparisons Streptomyces racemochromogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.4 Ca2+ required Streptomyces racemochromogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.4 55000
-
x * 55000, SDS-PAGE Streptomyces racemochromogenes

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Streptomyces racemochromogenes E0D7I2
-
-
3.1.4.4 Streptomyces racemochromogenes 10-3 E0D7I2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.4 native enzyme 17.4fold by ammonium sulfate fractionation, and cation and anion exchange chromatography Streptomyces racemochromogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.4 374.3
-
purified PLD103, pH 7.5, 37°C Streptomyces racemochromogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 phosphatidylcholine + H2O egg yolk lecithin Streptomyces racemochromogenes choline + phosphatidate
-
?
3.1.4.4 phosphatidylcholine + H2O egg yolk lecithin Streptomyces racemochromogenes 10-3 choline + phosphatidate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.4.4 ? x * 55000, SDS-PAGE Streptomyces racemochromogenes

Synonyms

EC Number Synonyms Comment Organism
3.1.4.4 PLD103
-
Streptomyces racemochromogenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.4 50
-
-
Streptomyces racemochromogenes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.4 10 60 over 80% of the activity remaining after 10 min within the temperature range Streptomyces racemochromogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.4 7.5
-
PLD103 exerts the highest hydrolytic activity at a slightly alkaline pH Streptomyces racemochromogenes

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.4.4 4 9 purified enzyme, over 70% activity remaining after 4 h Streptomyces racemochromogenes

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.4.4 Streptomyces racemochromogenes isoelectric focusing
-
8