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Literature summary extracted from

  • Okita, N.; Ohta, R.; Ashizawa, D.; Yamada, Y.; Abe, H.; Abe, T.; Tanuma, S.
    Bacterial production of recombinant human poly(ADP-ribose) glycohydrolase (2011), Protein Expr. Purif., 75, 230-235.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.143 the catalytic domain is expressed in Escherichia coli Rosetta2 (DE3) cells as a fusion protein with a glutathione S-transferase tag at the N-terminus and a hexahistidine tag at the C-terminus Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.143 adenosine diphosphate (hydroxymethyl) pyrrolidinediol
-
 Homo sapiens
3.2.1.143 eosin Y
-
 Homo sapiens
3.2.1.143 phloxine B
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.143 0.009
-
 Poly(ADP-ribose) purified recombinant catalytic enzyme domain, in 50 mM KPO4 (pH 7.2), at 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.143 90000
-
 x * 90000, purified recombinant catalytic enzyme domain, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.143 Homo sapiens Q86W56
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.143 Talon immobilized metal affinity resin column chromatography and glutathione-Sepharose column chromatography Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.143 35.6
-
 purified recombinant catalytic enzyme domain, at pH 7.2, 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.143 poly(ADP-ribose) + H2O
-
 Homo sapiens ADP-ribose oligomer + ADP-ribose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.143 ? x * 90000, purified recombinant catalytic enzyme domain, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.143 PARG
-
 Homo sapiens