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Literature summary extracted from

  • Okuyama, M.; Mori, H.; Chiba, S.; Kimura A.
    Overexpression and characterization of two unknown proteins, YicI and YihQ, originated from Escherichia coli (2004), Protein Expr. Purif., 37, 170-179.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.177
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.177 0.17
-
 alpha-D-xylopyranosyl fluoride pH 7.0, 37°C Escherichia coli
3.2.1.177 0.52
-
 4-nitrophenyl-alpha-D-xyloside pH 7.0, 37°C Escherichia coli
3.2.1.177 0.55
-
 Isoprimeverose pH 7.0, 37°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.177 88000
-
 6 * 88000, SDS-PAGE Escherichia coli
3.2.1.177 88079
-
 6 * 88079, calculated from sequence Escherichia coli
3.2.1.177 530000
-
 gel filtration Escherichia coli
3.2.1.199 70000
-
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.177 Escherichia coli P31434
-
-
3.2.1.199 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.177
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.177 0.503
-
 pH 7.0, 37°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.177 4-nitrophenyl-alpha-D-xyloside + H2O
-
 Escherichia coli 4-nitrophenol + alpha-D-xylose
-
 ?
3.2.1.177 alpha-D-xylopyranosyl fluoride + H2O
-
 Escherichia coli alpha-D-xylose + fluoride
-
 ?
3.2.1.177 isoprimeverose + H2O
-
 Escherichia coli alpha-D-xylose + beta-D-glucose
-
 ?
3.2.1.177 additional information the enzyme alos shows transglycosylation activity Escherichia coli ?
-
 ?
3.2.1.177 xyloglucan oligosaccharide + H2O
-
 Escherichia coli alpha-D-xylose + ?
-
 ?
3.2.1.199 additional information enzyme shows weak activities on isomaltose, D-glucosyl fluoride and 4-nitrophenyl alpha-glucoside, but the hydrolytic activities on maltose, kojibiose, nigerose, trehalose, isomaltotriose, glucose 1-phosphate, panose, alpha-maltosyl fluoride, alpha-xylosyl Xuoride, 4-nitrophenyl alpha-xyloside, and isoprimeverose are under detectable limits Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.177 hexamer 6 * 88000, SDS-PAGE Escherichia coli
3.2.1.177 hexamer 6 * 88079, calculated from sequence Escherichia coli
3.2.1.199 ? x * 77274, calculated, x * 70000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.2.1.177 YicI
-
 Escherichia coli
3.2.1.199 yihQ
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.177 47
-
 15 min, stable up to Escherichia coli
3.2.1.177 55
-
 15 min, about 70% loss of activity Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.177 0.173
-
 4-nitrophenyl-alpha-D-xyloside pH 7.0, 37°C Escherichia coli
3.2.1.177 51.3
-
 Isoprimeverose pH 7.0, 37°C Escherichia coli
3.2.1.177 195
-
 alpha-D-xylopyranosyl fluoride pH 7.0, 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.177 7
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.177 5 9 pH 5.0: about 35% of maximal activity, pH 9.0: about 65% of maximal activity Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.177 4.7 10.1 4°C, 24 h, stable Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.177 3.33
-
 4-nitrophenyl-alpha-D-xyloside pH 7.0, 37°C Escherichia coli
3.2.1.177 93.3
-
 Isoprimeverose pH 7.0, 37°C Escherichia coli
3.2.1.177 1220
-
 alpha-D-xylopyranosyl fluoride pH 7.0, 37°C Escherichia coli