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Literature summary extracted from

  • Heinzelman, P.; Komor, R.; Kanaan, A.; Romero, P.; Yu, X.; Mohler, S.; Snow, C.; Arnold, F.
    Efficient screening of fungal cellobiohydrolase class I enzymes for thermostabilizing sequence blocks by SCHEMA structure-guided recombination (2010), Protein Eng. Des. Sel., 23, 871-880.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.176 expressed in Saccharomyces cerevisiae strain YDR483W Trichoderma reesei
3.2.1.176 expressed in Saccharomyces cerevisiae strain YDR483W Thermoascus aurantiacus
3.2.1.176 expressed in Saccharomyces cerevisiae strain YDR483W Thermochaetoides thermophila
3.2.1.176 expressed in Saccharomyces cerevisiae strain YDR483W Rasamsonia emersonii
3.2.1.176 expressed in Saccharomyces cerevisiae strain YDR483W Acremonium thermophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.176 cellulose + H2O Trichoderma reesei
-
 ?
-
 ?
3.2.1.176 cellulose + H2O Thermoascus aurantiacus
-
 ?
-
 ?
3.2.1.176 cellulose + H2O Thermochaetoides thermophila
-
 ?
-
 ?
3.2.1.176 cellulose + H2O Rasamsonia emersonii
-
 ?
-
 ?
3.2.1.176 cellulose + H2O Acremonium thermophilum
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Acremonium thermophilum
-
-
-
3.2.1.176 Rasamsonia emersonii
-
-
-
3.2.1.176 Thermoascus aurantiacus
-
-
-
3.2.1.176 Thermochaetoides thermophila
-
-
-
3.2.1.176 Trichoderma reesei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.176 Ni2+ affinity column chromatography Trichoderma reesei
3.2.1.176 Ni2+ affinity column chromatography Thermoascus aurantiacus
3.2.1.176 Ni2+ affinity column chromatography Thermochaetoides thermophila
3.2.1.176 Ni2+ affinity column chromatography Rasamsonia emersonii
3.2.1.176 Ni2+ affinity column chromatography Acremonium thermophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 4-methylumbelliferyl lactoside + H2O
-
 Trichoderma reesei 4-methylumbelliferone + lactose
-
 ?
3.2.1.176 4-methylumbelliferyl lactoside + H2O
-
 Thermoascus aurantiacus 4-methylumbelliferone + lactose
-
 ?
3.2.1.176 4-methylumbelliferyl lactoside + H2O
-
 Thermochaetoides thermophila 4-methylumbelliferone + lactose
-
 ?
3.2.1.176 4-methylumbelliferyl lactoside + H2O
-
 Rasamsonia emersonii 4-methylumbelliferone + lactose
-
 ?
3.2.1.176 cellulose + H2O
-
 Trichoderma reesei ?
-
 ?
3.2.1.176 cellulose + H2O
-
 Thermoascus aurantiacus ?
-
 ?
3.2.1.176 cellulose + H2O
-
 Thermochaetoides thermophila ?
-
 ?
3.2.1.176 cellulose + H2O
-
 Rasamsonia emersonii ?
-
 ?
3.2.1.176 cellulose + H2O
-
 Acremonium thermophilum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.176 CBH I
-
 Trichoderma reesei
3.2.1.176 CBH I
-
 Thermoascus aurantiacus
3.2.1.176 CBH I
-
 Thermochaetoides thermophila
3.2.1.176 CBH I
-
 Rasamsonia emersonii
3.2.1.176 CBH I
-
 Acremonium thermophilum
3.2.1.176 cellobiohydrolase class I
-
 Trichoderma reesei
3.2.1.176 cellobiohydrolase class I
-
 Thermoascus aurantiacus
3.2.1.176 cellobiohydrolase class I
-
 Thermochaetoides thermophila
3.2.1.176 cellobiohydrolase class I
-
 Rasamsonia emersonii
3.2.1.176 cellobiohydrolase class I
-
 Acremonium thermophilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.176 65
-
 the enzyme is not active at temperatures higher than 65°C Trichoderma reesei
3.2.1.176 65
-
 the enzyme is not active at temperatures higher than 65°C Thermoascus aurantiacus
3.2.1.176 65
-
 the enzyme is not active at temperatures higher than 65°C Thermochaetoides thermophila
3.2.1.176 65
-
 the enzyme is not active at temperatures higher than 65°C Rasamsonia emersonii
3.2.1.176 65
-
 the enzyme is not active at temperatures higher than 65°C Acremonium thermophilum