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Literature summary extracted from
- Heterologous expression and characterization of the carboxylesterase from Geobacillus stearothermophilus (2010), Prog. Biochem. Biophys., 37, 967-974.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.1 | expressed in Pichia pastoris strain GS115 and Aspergillus niger strain M54 | Geobacillus stearothermophilus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 29000 | - |
x * 29000, recombinant His-tagged enzyme, SDS-PAGE | Geobacillus stearothermophilus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.1 | Geobacillus stearothermophilus | - |
- |
- |
| 3.1.1.1 | Geobacillus stearothermophilus CICC 20156 | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.1 | 1-naphthyl acetate + H2O | - |
Geobacillus stearothermophilus | 1-naphthol + acetate | - |
? |  |
| 3.1.1.1 | 1-naphthyl acetate + H2O | - |
Geobacillus stearothermophilus CICC 20156 | 1-naphthol + acetate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl acetate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + acetate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl acetate + H2O | - |
Geobacillus stearothermophilus CICC 20156 | 4-nitrophenol + acetate | - |
? | |
| 3.1.1.1 | cocaine + H2O | - |
Geobacillus stearothermophilus | O-benzoylecgonine + methanol | - |
? | |
| 3.1.1.1 | cocaine + H2O | - |
Geobacillus stearothermophilus CICC 20156 | O-benzoylecgonine + methanol | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.1 | ? | x * 29000, recombinant His-tagged enzyme, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 60 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 70 | - |
the recombinant enzyme shows 76.7-67.6% activity after incubation at 70°C for 30 min | Geobacillus stearothermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 8 | - |
- |
Geobacillus stearothermophilus |
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Release 2023.1 (January 2023)
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