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Literature summary extracted from

  • Sharma, K.; John, P.
    Purification and characterization of tannase and tannase gene from Enterobacter sp. (2011), Process Biochem., 46, 240-244.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.20 3.7
-
 methyl gallate pH and temperature not specified in the publication Enterobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.20 90000
-
 x * 90000, SDS-PAGE Enterobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.20 tannic acid + H2O Enterobacter sp.
-
 gallic acid + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O Enterobacter sp. KPJ03
-
 gallic acid + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Enterobacter sp. C7EDT0
-
-
3.1.1.20 Enterobacter sp. KPJ03 C7EDT0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 ammonium sulfate precipitation, DEAE cellulose column chromatography, and Sephadex G-100 gel filtration Enterobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.20 0.084
-
 crude extract, pH and temperature not specified in the publication Enterobacter sp.
3.1.1.20 13.63
-
 after 162fold purification, pH and temperature not specified in the publication Enterobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 methyl gallate + H2O
-
 Enterobacter sp. gallate + methanol
-
 ?
3.1.1.20 methyl gallate + H2O
-
 Enterobacter sp. KPJ03 gallate + methanol
-
 ?
3.1.1.20 tannic acid + H2O
-
 Enterobacter sp. gallic acid + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O
-
 Enterobacter sp. KPJ03 gallic acid + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.20 ? x * 90000, SDS-PAGE Enterobacter sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.1.20 tannin acyl hydrolase
-
 Enterobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 40
-
-
 Enterobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.20 60
-
 the enzyme retains 20% of its activity at 60°C Enterobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 5.5
-
-
 Enterobacter sp.

General Information

EC Number General Information Comment Organism
3.1.1.20 physiological function tannase and the organism itself are employed to protect grazing animals and environment against the toxic effects caused by tannins in them Enterobacter sp.