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Literature summary extracted from

  • Liu, J.; Tang, X.; Wang, B.; Yu, H.; Min, H.
    Cloning, screening and characterization of ester hydrolases with enantioselectivity in typical bacteria (2010), Process Biochem., 45, 475-480.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.12 expressed in Escherichia coli BL21(DE3) Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.12 Pseudomonas putida Q88MF4
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-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.12 4-nitrophenyl butyrate + H2O hydrolysis of chiral substrates (4-chloro-3-hydroxybutyl acetate, 1-phenylethyl acetate, methyl mandelate) observed, esterification with n-propanol of chiral substrates (ketoprofen, ibuprofen) observed, esterification with butyl acid of chiral substrate (2-octanol) observed Pseudomonas putida 4-nitrophenol + butyrate
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Synonyms

EC Number Synonyms Comment Organism
3.1.2.12 S-formylglutathione hydrolase
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Pseudomonas putida