EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.11 | expression as cellulose-binding module-intein-fusion protein in Escherichia coli strain BL21 Star (DE3) | Thermotoga maritima |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.11 | 0.04 | - |
D-fructose 1,6-bisphosphate | pH 7.5, 60°C, purified recombinant enzyme | Thermotoga maritima | |
3.1.3.11 | 0.12 | - |
D-fructose 1,6-bisphosphate | pH 7.5, 80°C, purified recombinant enzyme | Thermotoga maritima |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.11 | Mg2+ | required | Thermotoga maritima |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.11 | 28000 | - |
x * 28000, SDS-PAGE | Thermotoga maritima |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.11 | D-fructose 1,6-bisphosphate + H2O | Thermotoga maritima | - |
D-fructose 6-phosphate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.11 | Thermotoga maritima | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.11 | recombinant cellulose-binding module-intein-fusion enzyme 5.62fold from Escherichia coli strain BL21 Star (DE3) by affinity chromatography on regenerated amorphous cellulose followed by intein self-cleavage | Thermotoga maritima |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.11 | 12.13 | - |
pH 7.5, 60°C, purified recombinant enzyme | Thermotoga maritima |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.11 | D-fructose 1,6-bisphosphate + H2O | - |
Thermotoga maritima | D-fructose 6-phosphate + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.11 | ? | x * 28000, SDS-PAGE | Thermotoga maritima |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.11 | FBPase | - |
Thermotoga maritima |
3.1.3.11 | fructose-1,6-bisphosphatase | - |
Thermotoga maritima |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.11 | 80 | - |
- |
Thermotoga maritima |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.11 | 60 | - |
purified recombinant enzyme, half-life is 1330 h | Thermotoga maritima |
3.1.3.11 | 80 | - |
purified recombinant enzyme, half-life is 55.6 h | Thermotoga maritima |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.11 | 8.57 | - |
D-fructose 1,6-bisphosphate | pH 7.5, 60°C, purified recombinant enzyme | Thermotoga maritima | |
3.1.3.11 | 58.7 | - |
D-fructose 1,6-bisphosphate | pH 7.5, 80°C, purified recombinant enzyme | Thermotoga maritima |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.11 | 7.5 | - |
assay at | Thermotoga maritima |