EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.B16 | expression in Escherichia coli BL21 (DE3) | Taxus cuspidata |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.B16 | 0.0024 | - |
baccatin III | pH and temperature not specified in the publication | Taxus cuspidata | |
2.3.1.B16 | 0.0049 | - |
3-amino-3-phenylpropanoyl-CoA | pH and temperature not specified in the publication | Taxus cuspidata |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.B16 | 50000 | - |
x * 50000, SDS-PAGE | Taxus cuspidata |
2.3.1.B16 | 50546 | - |
x * 50546, calculated from sequence | Taxus cuspidata |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.B16 | baccatin III + 3-amino-3-phenylpropanoyl-CoA | Taxus cuspidata | the enzyme is involved in taxol biosynthesis | 3'-N-debenzoyl-2'-deoxytaxol + coenzyme A | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.B16 | Taxus cuspidata | Q8H2B5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.B16 | partial | Taxus cuspidata |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.B16 | baccatin III + 3-amino-3-phenylpropanoyl-CoA | the enzyme is involved in taxol biosynthesis | Taxus cuspidata | 3'-N-debenzoyl-2'-deoxytaxol + coenzyme A | - |
? | |
2.3.1.B16 | baccatin III + 3-amino-3-phenylpropanoyl-CoA | selective for 3-amino-3-phenylpropanoyl-CoA esters with a preference for beta-phenylalanoyl-CoA over 3-phenylisoserinoyl-CoA. alpha-Phenylalanoyl-CoA and N-benzoyl phenylisoserinoyl-CoA are not productive acyl donors | Taxus cuspidata | 3'-N-debenzoyl-2'-deoxytaxol + coenzyme A | this enzymatic product is converted to 2'-deoxytaxol by chemical N-benzoylation, and the identity of this derivative is confirmed by spectrometric analyses | ? | |
2.3.1.B16 | baccatin III + 3-phenylisoserinoyl-CoA | selective for 3-amino-3-phenylpropanoyl-CoA esters with a preference for beta-phenylalanoyl-CoA over 3-phenylisoserinoyl-CoA. Activity with 3-phenylisoserinoyl-CoA is 40% of the activity with beta-phenylalanoyl-CoA. alpha-Phenylalanoyl-CoA and N-benzoyl phenylisoserinoyl-CoA are not productive acyl donors | Taxus cuspidata | ? + coenzyme A | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.B16 | ? | x * 50000, SDS-PAGE | Taxus cuspidata |
2.3.1.B16 | ? | x * 50546, calculated from sequence | Taxus cuspidata |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.B16 | baccatin III 13-O-(3-amino-3-phenylpropanoyl)transferase | - |
Taxus cuspidata |
2.3.1.B16 | TAX7 | - |
Taxus cuspidata |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.B16 | 31 | - |
assay at | Taxus cuspidata |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.B16 | 6.8 | - |
recombinant enzyme | Taxus cuspidata |