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Literature summary extracted from

  • Cunningham, F.X. Jr.; Gantt, E.
    One ring or two? Determination of ring number in carotenoids by lycopene epsilon-cyclases (2001), Proc. Natl. Acad. Sci. USA, 98, 2905-2910.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.18 expression in Escherichia coli Lactuca sativa
5.5.1.18 expression in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
5.5.1.18 A447S like the wild-type enzyme the mutant enzyme adds two epsilon-rings to lycopene, forming epsilon-carotene Arabidopsis thaliana
5.5.1.18 H457D the mutant enzyme forms a higher amount of ratio delta-carotene compared to the wild-type enzyme Lactuca sativa
5.5.1.18 H457L the mutant adds only one epsilon-ring to lycopene, forming delta-carotene Lactuca sativa
5.5.1.18 H457R like the wild-type enzyme the mutant enzyme predominantly form epsilon-carotene (91%) Lactuca sativa
5.5.1.18 L448H the mutant adds two epsilon-ring to lycopene, forming epsilon-carotene Arabidopsis thaliana
5.5.1.18 L448R the mutant adds two epsilon-ring to lycopene, forming epsilon-carotene Arabidopsis thaliana
5.5.1.18 additional information construction and characterization of chimeric epsilon-cyclases of the epsilion-cyclases from Lactuca sativa and Arabidopsis thaliana Lactuca sativa
5.5.1.18 additional information construction and characterization of chimeric epsilon-cyclases of the epsilion-cyclases from Lactuca sativa and Arabidopsis thaliana Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.18 Arabidopsis thaliana Q38932
-
-
5.5.1.18 Lactuca sativa Q9AXK7
-
-
5.5.1.18 Lactuca sativa var. romaine Q9AXK7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.18 lycopene wild-type enzyme and mutant enzyme A447D acts one psi end of lycopene and forms monocyclic delta-carotene. Mutant enzymes L448H and L448R add two epsilon-ring to lycopene, forming epsilon-carotene Arabidopsis thaliana delta-carotene
-
 ?
5.5.1.18 lycopene the enzyme acts on both psi ends of lycopene, forming bicyclic epsilon-carotene. Mutant enzyme H457L adds only one epsilon-ring to lycopene, forming delta-carotene Lactuca sativa epsilon-carotene
-
 ?
5.5.1.18 lycopene the enzyme acts on both psi ends of lycopene, forming bicyclic epsilon-carotene. Mutant enzyme H457L adds only one epsilon-ring to lycopene, forming delta-carotene Lactuca sativa var. romaine epsilon-carotene
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.5.1.18 LCYe
-
 Lactuca sativa
5.5.1.18 LCYe
-
 Arabidopsis thaliana
5.5.1.18 lycopene bi-epsilon-cyclase
-
 Lactuca sativa