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Literature summary extracted from

  • Ling, J.; Soell, D.
    Severe oxidative stress induces protein mistranslation through impairment of an aminoacyl-tRNA synthetase editing site (2010), Proc. Natl. Acad. Sci. USA, 107, 4028-4033.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.3 hydrogen peroxide oxidizes cysteine182 residue critical for editing, which leads to Ser-tRNAThr formation and protein mistranslation that impaired growth of Escherichia coli. Presence of major heat shock proteases is required to allow cell growth in medium containing serine and hydrogen peroxide, which suggests that the mistranslated proteins are misfolded Escherichia coli
6.1.1.3 additional information reactive oxygen species cause editing defect and misacylation by WT ThrRS Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.3 additional information
-
additional information kinetics of diphosphate exchange activities and threonylation of tRNAThr of ThrRS with or without H2O2 treatment, overview Escherichia coli
6.1.1.3 0.0015
-
tRNAThr pH 7.2, 37°C, ThrRS, aminoacylation with Thr Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.3 Mg2+
-
Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.3 ATP + L-threonine + tRNAThr Escherichia coli
-
AMP + diphosphate + L-threonyl-tRNAThr
-
?
6.1.1.3 ATP + L-threonine + tRNAThr Escherichia coli MG1655
-
AMP + diphosphate + L-threonyl-tRNAThr
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.3 Escherichia coli
-
-
-
6.1.1.3 Escherichia coli MG1655
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.3 ATP + L-threonine + tRNAThr
-
Escherichia coli AMP + diphosphate + L-threonyl-tRNAThr
-
?
6.1.1.3 ATP + L-threonine + tRNAThr
-
Escherichia coli MG1655 AMP + diphosphate + L-threonyl-tRNAThr
-
?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.3 Threonyl-tRNA synthetase
-
Escherichia coli
6.1.1.3 ThrRS
-
Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.3 37
-
assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.3 0.05
-
tRNAThr pH 7.2, 37°C, ThrRS, aminoacylation with Thr Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.3 7.2
-
assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.3 ATP
-
Escherichia coli

General Information

EC Number General Information Comment Organism
6.1.1.3 malfunction reactive oxygen species cause editing defect and misacylation by WT ThrRS. H2O2-induced Ser-tRNAThr formation causes protein mistranslation Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.3 38.3
-
tRNAThr pH 7.2, 37°C, ThrRS, aminoacylation with Thr Escherichia coli