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Literature summary extracted from

  • Karanasios, E.; Han, G.S.; Xu, Z.; Carman, G.M.; Siniossoglou, S.
    A phosphorylation-regulated amphipathic helix controls the membrane translocation and function of the yeast phosphatidate phosphatase (2010), Proc. Natl. Acad. Sci. USA, 107, 17539-17544.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.4 additional information dephosphorylation of lipin Pah1p by the Nem1p-Spo7p complex enables the amphipathic helix to anchor Pah1p onto the nuclear/endoplasmic reticulum membrane allowing the production of diacylglycerol for lipid biosynthesis Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.4 0.43
-
 phosphatidic acid wild type enzyme, pH and temperature not specified in the publication Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.4 membrane recruitment of the yeast lipin (Pah1p) is regulated by PA levels onto the nuclear/endoplasmic reticulum membrane. Recruitment requires the transmembrane protein phosphatase complex Nem1p-Spo7p Saccharomyces cerevisiae 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.4 Mg2+ dependent on Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.4 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.4 PtA fusion affinity purification Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.4 phosphatidic acid + H2O
-
 Saccharomyces cerevisiae 1,2-diacyl-sn-glycerol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.4 lipin
-
 Saccharomyces cerevisiae
3.1.3.4 PA phosphatase
-
 Saccharomyces cerevisiae
3.1.3.4 Pah1p
-
 Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
3.1.3.4 physiological function lipins are essential regulators of fat metabolism, adipogenesis, and organelle biogenesis Saccharomyces cerevisiae