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Literature summary extracted from

  • Komatsu, M.; Tsuda, M.; Omura, S.; Oikawa. H.; Ikeda, H.
    Identification and functional analysis of genes controlling biosynthesis of 2-methylisoborneol (2008), Proc. Natl. Acad. Sci. USA, 105, 7422-7427.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.255 DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic analysis and tree, expression in Escherichia coli Saccharopolyspora erythraea
2.1.1.255 DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic analysis and tree, expression in Escherichia coli Streptomyces ambofaciens
2.1.1.255 DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic analysis and tree, expression in Escherichia coli Streptomyces lasalocidi
2.1.1.255 DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic analysis and tree, expression in Escherichia coli Micromonospora olivasterospora
2.1.1.255 expression in Escherichia coli Saccharopolyspora erythraea
2.1.1.255 expression in Escherichia coli Streptomyces ambofaciens
2.1.1.255 expression in Escherichia coli Streptomyces lasalocidi
4.2.3.118 expression in Streptomyces avermitilis Streptomyces lasalocidi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.118 Mg2+ required Streptomyces lasalocidi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.118 52400
-
 x * 52400, SDS-PAGE, His-tagged recombinant protein Streptomyces lasalocidi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Saccharopolyspora erythraea
-
 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Streptomyces ambofaciens
-
 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Streptomyces lasalocidi
-
 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Micromonospora olivasterospora
-
 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Saccharopolyspora erythraea the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Streptomyces ambofaciens the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Streptomyces lasalocidi the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Streptomyces lasalocidi NRRL2382R
-
 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Streptomyces lasalocidi NRRL2382R the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Micromonospora olivasterospora KY11048
-
 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Saccharopolyspora erythraea NRRL 2338
-
 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Saccharopolyspora erythraea NRRL 2338 the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Streptomyces ambofaciens ISP5053
-
 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate Streptomyces ambofaciens ISP5053 the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.255 Micromonospora olivasterospora F5HSX5
-
-
2.1.1.255 Micromonospora olivasterospora KY11048 F5HSX5
-
-
2.1.1.255 Saccharopolyspora erythraea A4FG18
-
-
2.1.1.255 Saccharopolyspora erythraea NRRL 2338 A4FG18
-
-
2.1.1.255 Streptomyces ambofaciens A3KI18
-
-
2.1.1.255 Streptomyces ambofaciens ISP5053 A3KI18
-
-
2.1.1.255 Streptomyces lasalocidi D3KYU3
-
-
2.1.1.255 Streptomyces lasalocidi NRRL2382R D3KYU3
-
-
4.2.3.118 Streptomyces lasalocidi D3KYU2
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate = S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate biosynthetic mechanism, overview Saccharopolyspora erythraea
a
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate = S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate biosynthetic mechanism, overview Streptomyces ambofaciens
a
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate = S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate biosynthetic mechanism, overview Streptomyces lasalocidi
a
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate = S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate biosynthetic mechanism, overview Micromonospora olivasterospora
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Saccharopolyspora erythraea S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Streptomyces ambofaciens S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Streptomyces lasalocidi S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Micromonospora olivasterospora S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Saccharopolyspora erythraea S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate GC-MS analysis of products ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Streptomyces ambofaciens S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate GC-MS analysis of products ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Streptomyces lasalocidi S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate GC-MS analysis of products ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Micromonospora olivasterospora S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate GC-MS analysis of products ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol Saccharopolyspora erythraea S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol Streptomyces ambofaciens S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol Streptomyces lasalocidi S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Streptomyces lasalocidi NRRL2382R S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Streptomyces lasalocidi NRRL2382R S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate GC-MS analysis of products ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol Streptomyces lasalocidi NRRL2382R S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Micromonospora olivasterospora KY11048 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Micromonospora olivasterospora KY11048 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate GC-MS analysis of products ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Saccharopolyspora erythraea NRRL 2338 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Saccharopolyspora erythraea NRRL 2338 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate GC-MS analysis of products ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol Saccharopolyspora erythraea NRRL 2338 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Streptomyces ambofaciens ISP5053 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate
-
 Streptomyces ambofaciens ISP5053 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate GC-MS analysis of products ?
2.1.1.255 S-adenosyl-L-methionine + geranyl diphosphate the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol Streptomyces ambofaciens ISP5053 S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
-
 ?
4.2.3.118 (E)-2-methylgeranyl diphosphate + H2O
-
 Streptomyces lasalocidi 2-methylisoborneol + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.118 ? x * 52400, SDS-PAGE, His-tagged recombinant protein Streptomyces lasalocidi

Synonyms

EC Number Synonyms Comment Organism
2.1.1.255 GPP methyltransferase
-
 Saccharopolyspora erythraea
2.1.1.255 GPP methyltransferase
-
 Streptomyces ambofaciens
2.1.1.255 GPP methyltransferase
-
 Streptomyces lasalocidi
4.2.3.118 2-MIB synthase
-
 Streptomyces lasalocidi

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.255 S-adenosyl-L-methionine
-
 Saccharopolyspora erythraea
2.1.1.255 S-adenosyl-L-methionine
-
 Streptomyces ambofaciens
2.1.1.255 S-adenosyl-L-methionine
-
 Streptomyces lasalocidi
2.1.1.255 S-adenosyl-L-methionine
-
 Micromonospora olivasterospora

General Information

EC Number General Information Comment Organism
2.1.1.255 metabolism the enzyme is important in the biosynthesis of 2-methylisoborneol, which involves the methylation of geranyl diphosphate by GPP methyltransferase and its subsequent cyclization by monoterpene cyclase to 2-methylisoborneol Saccharopolyspora erythraea
2.1.1.255 metabolism the enzyme is important in the biosynthesis of 2-methylisoborneol, which involves the methylation of geranyl diphosphate by GPP methyltransferase and its subsequent cyclization by monoterpene cyclase to 2-methylisoborneol Streptomyces ambofaciens
2.1.1.255 metabolism the enzyme is important in the biosynthesis of 2-methylisoborneol, which involves the methylation of geranyl diphosphate by GPP methyltransferase and its subsequent cyclization by monoterpene cyclase to 2-methylisoborneol Streptomyces lasalocidi
2.1.1.255 metabolism the enzyme is important in the biosynthesis of 2-methylisoborneol, which involves the methylation of geranyl diphosphate by GPP methyltransferase and its subsequent cyclization by monoterpene cyclase to 2-methylisoborneol Micromonospora olivasterospora
4.2.3.118 physiological function biosynthetic pathway to 2-methylisoborneol involves the methylation of geranyl diphosphate by GPP methyltransferase and its subsequent cyclization by monoterpene cyclase to 2-methylisoborneol Streptomyces lasalocidi