Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ogiwara, K.; Takahashi. T.
    Specificity of the medaka enteropeptidase serine protease and its usefulness as a biotechnological tool for fusion-protein cleavage (2007), Proc. Natl. Acad. Sci. USA, 104, 7021-7026.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.9 expression in Escherichia coli Oryzias latipes

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.9 E173A site directed mutagenesis Oryzias latipes
3.4.21.9 E173K site directed mutagenesis Oryzias latipes
3.4.21.9 F144A site directed mutagenesis Oryzias latipes
3.4.21.9 F144S site directed mutagenesis Oryzias latipes
3.4.21.9 K63A site directed mutagenesis Oryzias latipes
3.4.21.9 K63E site directed mutagenesis Oryzias latipes
3.4.21.9 K63R site directed mutagenesis Oryzias latipes
3.4.21.9 P193A site directed mutagenesis Oryzias latipes
3.4.21.9 P193E site directed mutagenesis Oryzias latipes
3.4.21.9 T105A site directed mutagenesis Oryzias latipes
3.4.21.9 T105E site directed mutagenesis Oryzias latipes

General Stability

EC Number General Stability Organism
3.4.21.9 no loss of activity at -20°C or 4°C for 6 months Oryzias latipes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.9 0.1
-
 Z-Phe-Arg-4-methylcoumaryl-7-amide 37°C, pH 7.4 Oryzias latipes
3.4.21.9 0.1
-
 Z-Phe-Arg-4-methylcoumaryl-7-amide K63R mutant, 37°C, pH 7.4 Oryzias latipes
3.4.21.9 0.1
-
 Z-Phe-Arg-4-methylcoumaryl-7-amide T105E mutant, 37°C, pH 7.4 Oryzias latipes
3.4.21.9 0.2
-
 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide K63R mutant, 37°C, pH 7.4 Oryzias latipes
3.4.21.9 0.2
-
 Z-Phe-Arg-4-methylcoumaryl-7-amide P193E mutant, 37°C, pH 7.4 Oryzias latipes
3.4.21.9 0.2
-
 Boc-Glu(OBzl)-Ala-Arg-MCA wild-type enzyme, 37°C, pH 7.4 Oryzias latipes
3.4.21.9 0.3
-
 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide E173A mutant, 37°C, pH 7.4 Oryzias latipes
3.4.21.9 0.4
-
 Z-Phe-Arg-4-methylcoumaryl-7-amide E173A mutant, 37°C, pH 7.4 Oryzias latipes
3.4.21.9 0.4
-
 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide P193E mutant, 37°C, pH 7.4 Oryzias latipes
3.4.21.9 0.4
-
 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide T105E mutant, 37°C, pH 7.4 Oryzias latipes
3.4.21.9 0.7
-
 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide 37°C, pH 7.4 Oryzias latipes
3.4.21.9 1
-
 Pro-Phe-Arg-4-methylcoumaryl-7-amide E173A mutant, 37°C, pH 7.4 Oryzias latipes
3.4.21.9 1
-
 Boc-Glu(OBzl)-Ala-Arg-MCA E173A mutant, 37°C, pH 7.4 Oryzias latipes
3.4.21.9 1
-
 Pro-Phe-Arg-4-methylcoumaryl-7-amide P193E mutant, 37°C, pH 7.4 Oryzias latipes
3.4.21.9 1
-
 Boc-Glu(OBzl)-Ala-Arg-MCA P193E mutant, 37°C, pH 7.4 Oryzias latipes
3.4.21.9 1.1
-
 Pro-Phe-Arg-4-methylcoumaryl-7-amide K63R mutant, 37°C, pH 7.4 Oryzias latipes
3.4.21.9 1.2
-
 Boc-Glu(OBzl)-Ala-Arg-MCA K63R mutant, 37°C, pH 7.4 Oryzias latipes
3.4.21.9 1.3
-
 Pro-Phe-Arg-4-methylcoumaryl-7-amide T105E mutant, 37°C, pH 7.4 Oryzias latipes
3.4.21.9 1.3
-
 Boc-Glu(OBzl)-Ala-Arg-MCA T105E mutant, 37°C, pH 7.4 Oryzias latipes
3.4.21.9 10
-
 Pro-Phe-Arg-4-methylcoumaryl-7-amide 37°C, pH 7.4 Oryzias latipes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.9 36000
-
 Western blot Oryzias latipes
3.4.21.9 44000
-
 10 * 44000, gel filtration Oryzias latipes

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.9 Oryzias latipes A4UWM5 medaka
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.9 of the recombinant fusion protein using nickel affinity column chromatography Oryzias latipes

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.9 intestine
-
 Oryzias latipes
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.9 Boc-Glu(OBzl)-Ala-Arg-4-methylcoumaryl-7-amide + H2O
-
 Oryzias latipes Boc-Glu(OBzl)-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.9 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O
-
 Oryzias latipes Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine
-
 ?
3.4.21.9 Pro-Phe-Arg-4-methylcoumaryl-7-amide + H2O
-
 Oryzias latipes 7-amino-4-methylcoumarin + Pro-Phe-Arg
-
 ?
3.4.21.9 trypsinogen + H2O
-
 Oryzias latipes trypsin + ?
-
 ?
3.4.21.9 Z-Phe-Arg-4-methylcoumaryl-7-amide + H2O
-
 Oryzias latipes 7-amino-4-methylcoumarin + Z-Phe-Arg
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.9 multimer 10 * 44000, gel filtration Oryzias latipes

Synonyms

EC Number Synonyms Comment Organism
3.4.21.9 enteropeptidase EP Oryzias latipes
3.4.21.9 EP-1
-
 Oryzias latipes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.9 37
-
 loss of 30% of activity after 4 days Oryzias latipes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.9 8
-
-
 Oryzias latipes