EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.1.99.17 | chloroplast | - |
Arabidopsis thaliana | 9507 | - |
4.1.99.17 | plastid | - |
Arabidopsis thaliana | 9536 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.99.17 | iron-sulfur centre | - |
Arabidopsis thaliana |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.99.17 | 71994 | - |
x * 71994, calculated from sequence | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.99.17 | 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine | Arabidopsis thaliana | the enzyme is involved in the biosynthesis of thiamine diphosphate | 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO | - |
? | |
4.1.99.17 | 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine | Arabidopsis thaliana ecotype Col-0 | the enzyme is involved in the biosynthesis of thiamine diphosphate | 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.99.17 | Arabidopsis thaliana | O82392 | - |
- |
4.1.99.17 | Arabidopsis thaliana ecotype Col-0 | O82392 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.1.99.17 | seedling | THIC transcript is not detectable two days after germination, but is readily detectable at day five | Arabidopsis thaliana | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.99.17 | 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO | - |
? | |
4.1.99.17 | 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine | the enzyme is involved in the biosynthesis of thiamine diphosphate | Arabidopsis thaliana | 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO | - |
? | |
4.1.99.17 | 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine | - |
Arabidopsis thaliana ecotype Col-0 | 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO | - |
? | |
4.1.99.17 | 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine | the enzyme is involved in the biosynthesis of thiamine diphosphate | Arabidopsis thaliana ecotype Col-0 | 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.99.17 | ? | x * 71994, calculated from sequence | Arabidopsis thaliana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.99.17 | thiC | - |
Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.99.17 | iron-sulfur centre | - |
Arabidopsis thaliana |
EC Number | Organism | Comment | Expression |
---|---|---|---|
4.1.99.17 | Arabidopsis thaliana | the THIC gene is negatively regulated by thiamin itself, regulation by conserved regions of mRNA that bind specific metabolites (riboswitches). As the thiamine riboswitch only responds to thiamin diphosphate but not thiamine, the down-regulation of THIC mRNA implies that the externally provided thiamine is converted to thiamine diphosphate inside the cell leading to the conformational change inducing mRNA instability | down |
4.1.99.17 | Arabidopsis thaliana | the transcript level in seedlings grown in 24 h of light is substantially higher than that in seedlings exposed to a single long day cycle (16-h light/8-h dark) | up |
4.1.99.17 | Arabidopsis thaliana | THIC transcript is not detectable two days after germination, but is readily detectable at day five | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.1.99.17 | malfunction | knockdown mutant, if the thiC plants are not supplemented with thiamine, they eventually die. A concentration of 1.5 M thiamine is sufficient to allow growth of the seedlings, but these are chlorotic | Arabidopsis thaliana |
4.1.99.17 | physiological function | the enzyme is involved in the biosynthesis of thiamine diphosphate, the enzyme is essential for plant viability | Arabidopsis thaliana |