Literature summary extracted from
Lin, R.; Wu, R.; Huang, X.; Xie, T.
Immobilization of oxalate decarboxylase to Eupergit and properties of the immobilized enzyme (2011), Prep. Biochem. Biotechnol., 41, 154-165.
General Stability
EC Number |
General Stability |
Organism |
---|
4.1.1.2 |
under the condition of enzyme/support ratio at 1:20, pH 9, with 1.5 M (NH4)2SO4, 22°C, and shaking at 30 rpm for 24 h, activity recovery of Eupergit C-immobilized enzyme reaches 90% |
Bacillus subtilis |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
4.1.1.2 |
15.46 |
- |
oxalate |
soluble enzyme, citrate buffer (0.05 M, pH 4.0), at 37°C |
Bacillus subtilis |
|
4.1.1.2 |
22.61 |
- |
oxalate |
Eupergit C-immobilized enzyme, citrate buffer (0.05 M, pH 4.0), at 37°C |
Bacillus subtilis |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.1.1.2 |
Mn2+ |
Mn2+ binding to the enzyme is not easy to release |
Bacillus subtilis |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.1.1.2 |
Bacillus subtilis |
- |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.1.1.2 |
oxalate + H+ |
- |
Bacillus subtilis |
formate + CO2 |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.1.1.2 |
OXDC |
- |
Bacillus subtilis |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
4.1.1.2 |
50 |
- |
soluble and immobilized enzyme |
Bacillus subtilis |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
4.1.1.2 |
30 |
70 |
the soluble enzyme shows at least more than 50% activity between 30 and 70°C. The immobilized enzyme almost retains its full maximum activity at 70°C (7% decrease of activity), while the soluble enzyme shows about 50% activity at 70°C |
Bacillus subtilis |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
4.1.1.2 |
60 |
70 |
both soluble and immobilized enzyme lose their activity continuously at 60°C treatment (1 h at pH 4.0). After thermal treatment for 10 min, 46.07% activity remains for the immobilized enzyme an keep 13.86% activity after 1 h of thermal treatment, while the soluble one loses its activity completely |
Bacillus subtilis |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
4.1.1.2 |
3.5 |
- |
soluble and immobilized enzyme |
Bacillus subtilis |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
4.1.1.2 |
3 |
5 |
soluble and immobilized enzyme. When decreasing pH to 3.0, the soluble enzyme loses 76% of its original activity, but there is only 41% loss for the immobilized enzyme |
Bacillus subtilis |
pH Stability
EC Number |
pH Stability |
pH Stability Maximum |
Comment |
Organism |
---|
4.1.1.2 |
3 |
- |
both the soluble and immobilized enzyme are stable after 6 h of treatment with buffer at a pH higher than 4.5, and no obvious activity decrease are observed. There is 36.9% activity preserved in the immobilized enzyme after 6 h of treatment with a pH 3.5 buffer, and 4.2% activity remains in the soluble enzyme |
Bacillus subtilis |