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Literature summary extracted from

  • Schmidl, S.R.; Otto, A.; Lluch-Senar, M.; Pinol, J.; Busse, J.; Becher, D.; Stuelke, J.
    A trigger enzyme in Mycoplasma pneumoniae: impact of the glycerophosphodiesterase GlpQ on virulence and gene expression (2011), PLoS Pathog., 7, e1002263.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.2 genes glpQ and mpn566, expression of N-terminally Strep-tagged enzymes Mycoplasma pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.2 additional information generationof GlpQ and of MPN566 knockout strains, phenotypes, overview. The MPN566-deficient strain does not show an altered phenotype Mycoplasma pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.2 Ca2+ no activity with Mycoplasma pneumoniae
3.1.4.2 Co2+ no activity with Mycoplasma pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.2 Mg2+ activates, required for activity Mycoplasma pneumoniae
3.1.4.2 Mn2+ activates, less activating than Mg2+ Mycoplasma pneumoniae
3.1.4.2 Zn2+ activates, less activating than Mg2+ Mycoplasma pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.2 sn-glycero-3-phosphocholine + H2O Mycoplasma pneumoniae
-
 choline + sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.2 Mycoplasma pneumoniae
-
 gene glpQ
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.2 N-terminally Strep-tagged enzymes Mycoplasma pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.2 sn-glycero-3-phosphocholine + H2O
-
 Mycoplasma pneumoniae choline + sn-glycerol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.2 GlpQ
-
 Mycoplasma pneumoniae
3.1.4.2 glycerophosphodiesterase
-
 Mycoplasma pneumoniae
3.1.4.2 MPN420
-
 Mycoplasma pneumoniae

General Information

EC Number General Information Comment Organism
3.1.4.2 malfunction inactivation of the glpQ gene results in reduced growth in medium with glucose as the carbon source, in loss of hydrogen peroxide production when phosphatidylcholine is present, and in a complete loss of cytotoxicity towards HeLa cells, and the glpQ mutant strain exhibited a reduced gliding velocity, overview. The phenotype is reversible par complementation with the wild-type enzyme Mycoplasma pneumoniae
3.1.4.2 additional information a second glycerophosphodiesterase enzyme, MPN566, is catalytically inactive and its deletion does not cause an altered phenotype Mycoplasma pneumoniae
3.1.4.2 physiological function GlpQ is implicated in the control of gene expression, apparent regulation by GlpQ is exerted at the level of transcription. All genes subject to GlpQ-dependent control have a conserved potential cis-acting element upstream of the coding region. This element overlaps the promoter in the case of the genes that are repressed in a GlpQ-dependent manner and it is located upstream of the promoter for GlpQ-activated genes. GlpQ is central to the normal physiology and to pathogenicity of the minimal pathogen Mycoplasma pneumoniae