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Literature summary extracted from
- Alanine racemase mutants of Burkholderia pseudomallei and Burkholderia mallei and use of alanine racemase as a non-antibiotic-based selectable marker (2011), PLoS ONE, 6, e21523.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.1.1 | expressed in Escherichia coli strain ALA1 | Burkholderia mallei |
| 5.1.1.1 | expressed in Escherichia coli strain ALA1 | Burkholderia pseudomallei |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.1.1 | Burkholderia mallei | Q62JA4 | - |
- |
| 5.1.1.1 | Burkholderia mallei ATCC 23344 | Q62JA4 | - |
- |
| 5.1.1.1 | Burkholderia pseudomallei | Q63ME6 | alanine racemase isoform 2 | - |
| 5.1.1.1 | Burkholderia pseudomallei | Q63SZ2 | alanine racemase isoform 1 | - |
| 5.1.1.1 | Burkholderia pseudomallei K96243 | Q63ME6 | alanine racemase isoform 2 | - |
| 5.1.1.1 | Burkholderia pseudomallei K96243 | Q63SZ2 | alanine racemase isoform 1 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.1.1 | L-alanine | - |
Burkholderia mallei | D-alanine | - |
? |  |
| 5.1.1.1 | L-alanine | - |
Burkholderia pseudomallei | D-alanine | - |
? | |
| 5.1.1.1 | L-alanine | - |
Burkholderia mallei ATCC 23344 | D-alanine | - |
? | |
| 5.1.1.1 | L-alanine | - |
Burkholderia pseudomallei K96243 | D-alanine | - |
? | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.1.1 | pyridoxal 5'-phosphate | - |
Burkholderia mallei | |
| 5.1.1.1 | pyridoxal 5'-phosphate | - |
Burkholderia pseudomallei | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.1.1.1 | malfunction | during log phase growth without D-alanine, the viable counts of alanine racemase-deficient mutants of Burkholderia pseudomallei decrease within 2 h by about 1000fold, and no viable bacteria are present at 24 h. The alanine racemase-deficient mutant of Burkholderia pseudomallei K96243 exhibits attenuation versus its isogenic parental strain with respect to growth and survival in murine peritoneal macrophages | Burkholderia pseudomallei |
| 5.1.1.1 | malfunction | during log phase growth without D-alanine, the viable counts of alanine racemase-deficient mutants of Burkholderia pseudomallei decrease within 2 h by about 10fold, and no viable bacteria are present at 24 h | Burkholderia mallei |
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Release 2023.1 (January 2023)
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