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Literature summary extracted from

  • Ramos, E.; Torre, E.A.; Bushey, A.M.; Gurudatta, B.V.; Corces, V.G.
    DNA topoisomerase II modulates insulator function in Drosophila (2011), PLoS ONE, 6, e16562.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.2.2 expression of the enzyme in a two-hybrid system with Mod(mdg4)2.2 proteins Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
5.6.2.2 additional information downregulation of Topo II by RNAi or mutations in the Top2 gene, knockout mutant phenotypes, overview. The Top2 RNAi knockdown affects gypsy phenotypes Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.2.2 chromosome Topo II and insulator proteins do not co-localize on polytene chromosomes Drosophila melanogaster 5694
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5.6.2.2 nucleus
-
 Drosophila melanogaster 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.2.2 additional information Drosophila melanogaster Topo II and Mod(mdg4)2.2 proteins directly interact ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.2 Drosophila melanogaster
-
 gene Top2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.2 additional information Topo II and Mod(mdg4)2.2 proteins directly interact Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.2.2 DNA topoisomerase II
-
 Drosophila melanogaster
5.6.2.2 topo II
-
 Drosophila melanogaster

General Information

EC Number General Information Comment Organism
5.6.2.2 malfunction downregulation of Topo II by RNAi or mutations in the Top2 gene result in disruption of Su(Hw) insulator function, mediated by the Mod(mdg4)2.2 protein, which is a unique component of the Su(Hw) insulator complex. Mutations in Top2 cause a slight decrease of Mod(mdg4)2.2 transcript but have a dramatic effect on Mod(mdg4)2.2 protein levels. Mod(mdg4)2.2 is not present at insulator sites in Top2 mutants, while Su(Hw) is present Drosophila melanogaster
5.6.2.2 physiological function Topo II is required to prevent Mod(mdg4)2.2 degradation and, consequently, to stabilize Su(Hw) insulator-mediated chromatin organization Drosophila melanogaster