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Literature summary extracted from

  • Utsumi, Y.; Utsumi, C.; Sawada, T.; Fujita, N.; Nakamura, Y.
    Functional diversity of isoamylase oligomers: the ISA1 homo-oligomer is essential for amylopectin biosynthesis in rice endosperm (2011), Plant Physiol., 156, 61-77.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.68 expressed in Escherichia coli BL21(DE3) Star cells Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.68 soluble
-
 Oryza sativa
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.68 Oryza sativa
-
 cultivar Kinmaze
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.68 HitrapQ column chromatography and TSKgel DEAE-5PW gel filtration Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.68 endosperm
-
 Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.68 glycogen + H2O
-
 Oryza sativa maltose + maltooligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.68 homooligomer
-
 Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
3.2.1.68 ISA1
-
 Oryza sativa
3.2.1.68 ISA2
-
 Oryza sativa

General Information

EC Number General Information Comment Organism
3.2.1.68 physiological function the isoamylase 1 is essential for amylopectin biosynthesis and starch production in rice endosperm. Overexpression of the isoamylase 2 gene brings about a dramatic reduction in kernel size in the dry seed and the transformants contain less than 50% of the starch in the kernel, while the content of soluble sugars, including maltodextrins, malto-oligosaccharides, and simple sugars, increase by about 8fold when compared with the host cultivar Kinmaze Oryza sativa