Literature summary extracted from
Sobotka, R.; Tichy, M.; Wilde, A.; Hunter, C.N.
Functional assignments for the carboxyl-terminal domains of the ferrochelatase from Synechocystis PCC 6803: the CAB domain plays a regulatory role, and region II is essential for catalysis (2011), Plant Physiol., 155, 1735-1747.
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
4.98.1.1 |
additional information |
deletion of the CAB domain prevents the DELTAH347 ferrochelatase mutant strain from growing at higher light intensities and affects the cellular accumulation of tetrapyrroles. The mutant is active as a monomer |
Synechocystis sp. |
General Stability
EC Number |
General Stability |
Organism |
---|
4.98.1.1 |
region II is critical for both the stability and enzyme activity of Synechocystis ferrochelatase |
Synechocystis sp. |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.98.1.1 |
Synechocystis sp. |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.98.1.1 |
anti-FLAG M2 affinity gel column chromatography or Ni2+-charged His-select resin column chromatography |
Synechocystis sp. |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.98.1.1 |
protoporphyrin IX + Zn2+ |
- |
Synechocystis sp. |
Zn-protoporphyrin IX + H+ |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.98.1.1 |
dimer |
full-length enzyme, the CAB domain is necessary for the dimerization of Synechocystis ferrochelatase in vivo |
Synechocystis sp. |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.98.1.1 |
FeCH |
- |
Synechocystis sp. |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
4.98.1.1 |
physiological function |
the apparent surplus of ferrochelatase activity in the wild type is critical for cell viability under high light due to a regulatory role of ferrochelatase in the distribution of chlorophyll into apoproteins |
Synechocystis sp. |