EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.3.87 | expressed in Escherichia coli BL21 (DE3) Codon Plus-RIL cells | Aquilaria crassna |
4.2.3.93 | expressed in Escherichia coli BL21 (DE) Codon plus-RIL cells | Aquilaria crassna |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.3.87 | W144R/V241E/P296S/H337Y | inactive | Aquilaria crassna |
4.2.3.87 | Y110S/V241E/P296S/H337Y | the mutant produces 34.1% alpha-guaiene, 1.3% alpha-humulene and 64.6% delta-guaiene | Aquilaria crassna |
4.2.3.87 | Y110S/W144R/P296S/H337Y | inactive | Aquilaria crassna |
4.2.3.87 | Y110S/W144R/V241E/H337Y | inactive | Aquilaria crassna |
4.2.3.87 | Y110S/W144R/V241E/P296S | inactive | Aquilaria crassna |
4.2.3.87 | Y110S/W144R/V241E/P296S/H337Y | the mutant produces 31.1% alpha-guaiene, 1.5% alpha-humulene and 67.4% delta-guaiene | Aquilaria crassna |
4.2.3.93 | Y110S/V241E/P296S/H337Y | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Aquilaria crassna |
4.2.3.93 | Y110S/W144R/V241E/P296S/H337Y | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Aquilaria crassna |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.93 | 0.000685 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme Y110S/V241E/P296S/H337Y, in Tris-HCl (25 mM, pH 7.0), at 30°C | Aquilaria crassna | |
4.2.3.93 | 0.00271 | - |
(2E,6E)-farnesyl diphosphate | wild type enzyme, in 50 mM Tris-HCl, pH 7.5, at 30°C | Aquilaria crassna | |
4.2.3.93 | 0.00429 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme Y110S/W144R/V241E/P296S/H337Y, in Tris-HCl (25 mM, pH 7.0), at 30°C | Aquilaria crassna |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.3.87 | Aquilaria crassna | - |
- |
- |
4.2.3.93 | Aquilaria crassna | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.3.87 | Ni-NTA column chromatography, gel filtration | Aquilaria crassna |
4.2.3.93 | Ni-NTA column chromatography | Aquilaria crassna |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.2.3.87 | leaf | - |
Aquilaria crassna | - |
4.2.3.93 | cell culture | - |
Aquilaria crassna | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.87 | (2E,6E)-farnesyl diphosphate | - |
Aquilaria crassna | alpha-guaiene + diphosphate | 13% yield | ? | |
4.2.3.93 | (2E,6E)-farnesyl diphosphate | - |
Aquilaria crassna | delta-guaiene + diphosphate | delta-guaiene is the major product (61% yield) | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.3.87 | TPS | - |
Aquilaria crassna |
4.2.3.93 | TPS | - |
Aquilaria crassna |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.93 | 0.000586 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme Y110S/W144R/V241E/P296S/H337Y, in Tris-HCl (25 mM, pH 7.0), at 30°C | Aquilaria crassna | |
4.2.3.93 | 0.00125 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme Y110S/V241E/P296S/H337Y, in Tris-HCl (25 mM, pH 7.0), at 30°C | Aquilaria crassna | |
4.2.3.93 | 0.00499 | - |
(2E,6E)-farnesyl diphosphate | wild type enzyme, in 50 mM Tris-HCl, pH 7.5, at 30°C | Aquilaria crassna |
EC Number | Organism | Comment | Expression |
---|---|---|---|
4.2.3.87 | Aquilaria crassna | the production of alpha-guaiene is induced by treatment with methyl jasmonate | up |
4.2.3.93 | Aquilaria crassna | the production of delta-guaiene is induced by treatment with 300 mM methyl jasmonate reaching its maximum after 12 h | up |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.93 | 0.137 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme Y110S/W144R/V241E/P296S/H337Y, in Tris-HCl (25 mM, pH 7.0), at 30°C | Aquilaria crassna | |
4.2.3.93 | 0.182 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme Y110S/V241E/P296S/H337Y, in Tris-HCl (25 mM, pH 7.0), at 30°C | Aquilaria crassna | |
4.2.3.93 | 1.836 | - |
(2E,6E)-farnesyl diphosphate | wild type enzyme, in 50 mM Tris-HCl, pH 7.5, at 30°C | Aquilaria crassna |