Any feedback?
Literature summary extracted from
- Characterization of plant carotenoid cyclases as members of the flavoprotein family functioning with no net redox change (2010), Plant Physiol., 153, 970-979.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.99.8 | expressed in Escherichia coli TOPO10 cells | Capsicum annuum |
| 5.5.1.19 | expressed in Escherichia coli TOPO10 cells | Capsicum annuum |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.3.99.8 | E295A | the mutation completely abolishes the formation of beta-carotene and kappa-cyclic carotenoids by CCS | Capsicum annuum |
| 5.3.99.8 | E295K | the mutation completely abolishes the formation of beta-carotene and kappa-cyclic carotenoids by CCS | Capsicum annuum |
| 5.3.99.8 | E295R | the mutation completely abolishes the formation of beta-carotene and kappa-cyclic carotenoids by CCS | Capsicum annuum |
| 5.3.99.8 | H360A | the mutant shows completely abolished activity | Capsicum annuum |
| 5.3.99.8 | H360K | the mutant shows partially recovered activity | Capsicum annuum |
| 5.3.99.8 | H360R | the mutant shows partially recovered activity | Capsicum annuum |
| 5.5.1.19 | D127A | the mutation drastically reduces the lycopene cyclase activity of CCS up to 90% | Capsicum annuum |
| 5.5.1.19 | D259A | the mutation drastically reduces the lycopene cyclase activity of CCS up to 90% | Capsicum annuum |
| 5.5.1.19 | E128A | the mutation drastically reduces the lycopene cyclase activity of CCS up to 90% | Capsicum annuum |
| 5.5.1.19 | E296A | the mutation reduces the lycopene cyclase activity of CCS | Capsicum annuum |
| 5.5.1.19 | E332A | the mutation drastically reduces the lycopene cyclase activity of CCS up to 90% | Capsicum annuum |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.3.99.8 | chromoplast | - |
Capsicum annuum | 9509 | - |
| 5.5.1.19 | chromoplast | - |
Capsicum annuum | 9509 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.99.8 | 66000 | - |
1 * 66000, SDS-PAGE | Capsicum annuum |
| 5.5.1.19 | 66000 | - |
1 * 66000, SDS-PAGE | Capsicum annuum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.99.8 | Capsicum annuum | Q42435 | - |
- |
| 5.5.1.19 | Capsicum annuum | Q42435 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.99.8 | Q-Sepharose column chromatography, Affigel 501 gel filtration, Mono-P column chromatography, and Sephacryl gel filtration, and Superose 6 gel filtration | Capsicum annuum |
| 5.5.1.19 | Q-Sepharose column chromatography, Affigel 501 gel filtration, Mono-P column chromatography, and Sephacryl gel filtration, and Superose 6 gel filtration | Capsicum annuum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.99.8 | antheraxanthin | - |
Capsicum annuum | capsanthin + 5,6-diepikarpoxanthin | - |
? |  |
| 5.3.99.8 | additional information | CCS is mainly dedicated to the synthesis of kappa-cyclic carotenoids (capsanthin and capsorubin) but also has lycopene beta-cyclase activity | Capsicum annuum | ? | - |
? | |
| 5.3.99.8 | violaxanthin | - |
Capsicum annuum | capsorubin + capsanthin-5,6-epoxide + 5,6-diepilatoxanthin | - |
? | |
| 5.5.1.19 | additional information | CCS is mainly dedicated to the synthesis of kappa-cyclic carotenoids (capsanthin and capsorubin) but also has lycopene beta-cyclase activity | Capsicum annuum | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.99.8 | monomer | 1 * 66000, SDS-PAGE | Capsicum annuum |
| 5.5.1.19 | monomer | 1 * 66000, SDS-PAGE | Capsicum annuum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.99.8 | capsanthin-capsorubin synthase | - |
Capsicum annuum |
| 5.3.99.8 | CCS | - |
Capsicum annuum |
| 5.5.1.19 | capsanthin-capsorubin synthase | - |
Capsicum annuum |
| 5.5.1.19 | CCS | - |
Capsicum annuum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.99.8 | FAD | the enzyme contains one noncovalently bound FAD that is essential for enzyme activity only in the presence of NADPH, which functions as the FAD reductant | Capsicum annuum | |
| 5.3.99.8 | NADPH | the enzyme contains one noncovalently bound FAD that is essential for enzyme activity only in the presence of NADPH, which functions as the FAD reductant | Capsicum annuum | |
| 5.5.1.19 | FAD | the enzyme contains one noncovalently bound FAD that is essential for enzyme activity only in the presence of NADPH, which functions as the FAD reductant | Capsicum annuum | |
| 5.5.1.19 | NADPH | the enzyme contains one noncovalently bound FAD that is essential for enzyme activity only in the presence of NADPH, which functions as the FAD reductant | Capsicum annuum | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
