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Literature summary extracted from

  • Huang, M.; Abel, C.; Sohrabi, R.; Petri, J.; Haupt, I.; Cosimano, J.; Gershenzon, J.; Tholl, D.
    Variation of herbivore-induced volatile terpenes among Arabidopsis ecotypes depends on allelic differences and subcellular targeting of two terpene synthases, TPS02 and TPS03 (2010), Plant Physiol., 153, 1293-1310.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.46 0.0026
-
 (2E,6E)-farnesyl diphosphate accession Columbia, 30°C, pH not specified in the publication Arabidopsis thaliana
4.2.3.46 0.004
-
 (2E,6E)-farnesyl diphosphate accession Wassilewskija, 30°C, pH not specified in the publication Arabidopsis thaliana
4.2.3.106 0.0026
-
 (2E,6E)-farnesyl diphosphate accession Columbia, 30°C, pH not specified in the publication Arabidopsis thaliana
4.2.3.106 0.0067
-
 geranyl diphosphate accession Columbia, 30°C, pH not specified in the publication Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.46 chloroplast plastidial transit peptide of 25 amino acids, and fluoresence studies using GPF-fusion protein, accession Wassilewskija Arabidopsis thaliana 9507
-
4.2.3.46 cytosol
-
 Arabidopsis thaliana 5829
-
4.2.3.106 cytosol
-
 Arabidopsis thaliana 5829
-

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.46 Arabidopsis thaliana A4FVP2 isoform TPS03
-
4.2.3.46 Arabidopsis thaliana P0CJ43 isoform TPS02
-
4.2.3.106 Arabidopsis thaliana A4FVP2 isoform TPS03
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.46 flower expression occurs constitutively in floral tissues Arabidopsis thaliana
-
4.2.3.106 flower expression occurs constitutively in floral tissues Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.46 (2E,6E)-farnesyl diphosphate
-
 Arabidopsis thaliana (3E,6E)-alpha-farnesene + diphosphate
-
 ?
4.2.3.46 additional information enzyme shows both (E)-beta-ocimene and (E,E)-alpha-farnesene synthase activities Arabidopsis thaliana ?
-
 ?
4.2.3.106 (2E,6E)-farnesyl diphosphate
-
 Arabidopsis thaliana (E)-beta-ocimene + diphosphate
-
 ?
4.2.3.106 geranyl diphosphate
-
 Arabidopsis thaliana (E)-beta-ocimene + diphosphate
-
 ?
4.2.3.106 additional information enzyme shows both (E)-beta-ocimene and (E,E)-alpha-farnesene synthase activities Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.46 TPS02
-
 Arabidopsis thaliana
4.2.3.46 TPS03
-
 Arabidopsis thaliana
4.2.3.106 TPS03
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.46 0.0045
-
 (2E,6E)-farnesyl diphosphate accession Wassilewskija, 30°C, pH not specified in the publication Arabidopsis thaliana
4.2.3.46 0.0048
-
 (2E,6E)-farnesyl diphosphate accession Columbia, 30°C, pH not specified in the publication Arabidopsis thaliana
4.2.3.106 0.0048
-
 (2E,6E)-farnesyl diphosphate accession Columbia, 30°C, pH not specified in the publication Arabidopsis thaliana
4.2.3.106 0.034
-
 geranyl diphosphate accession Columbia, 30°C, pH not specified in the publication Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
4.2.3.46 Arabidopsis thaliana expression is induced in leaves by elicitor and insect treatment up
4.2.3.106 Arabidopsis thaliana expression is induced in leaves by elicitor and insect treatment up

General Information

EC Number General Information Comment Organism
4.2.3.46 physiological function differences in the relative emissions of (E)-beta-ocimene and (E,E)-alpha-farnesene from accession Wassilewskija, a high-(E)-beta-ocimene emitter, and accession Columbia, a trace-(E)-beta-ocimene emitter, are attributed to allelic variation of closely related, tandem-duplicated terpene synthase genes, TPS02 and TPS03. The Wassilewskija genome contains a functional allele of TPS02 but not of TPS03, while the opposite is the case for Columbia. Recombinant proteins of the functional Wassilewskija TPS02 and Columbia TPS03 genes both show (E)-beta-ocimene and (E,E)-alpha-farnesene synthase activities. Differential subcellular compartmentalization of the two enzymes in plastids and the cytosol is responsible for the ecotype-specific differences in (E)-beta-ocimene/(E,E)-alpha-farnesene emission Arabidopsis thaliana
4.2.3.106 physiological function differences in the relative emissions of (E)-beta-ocimene and (E,E)-alpha-farnesene from accession Wassilewskija, a high-(E)-beta-ocimene emitter, and accession Columbia, a trace-(E)-beta-ocimene emitter, are attributed to allelic variation of closely related, tandem-duplicated terpene synthase genes, TPS02 and TPS03. The Wassilewskija genome contains a functional allele of TPS02 but not of TPS03, while the opposite is the case for Columbia. Recombinant proteins of the functional Wassilewskija TPS02 and Columbia TPS03 genes both show (E)-beta-ocimene and (E,E)-alpha-farnesene synthase activities. Differential subcellular compartmentalization of the two enzymes in plastids and the cytosol is responsible for the ecotype-specific differences in (E)-beta-ocimene/(E,E)-alpha-farnesene emission Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.3.46 1.12
-
 (2E,6E)-farnesyl diphosphate accession Wassilewskija, 30°C, pH not specified in the publication Arabidopsis thaliana
4.2.3.46 1.82
-
 (2E,6E)-farnesyl diphosphate accession Columbia, 30°C, pH not specified in the publication Arabidopsis thaliana
4.2.3.106 1.82
-
 (2E,6E)-farnesyl diphosphate accession Columbia, 30°C, pH not specified in the publication Arabidopsis thaliana
4.2.3.106 5.12
-
 geranyl diphosphate accession Columbia, 30°C, pH not specified in the publication Arabidopsis thaliana