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Literature summary extracted from

  • Schmidlin, L.; Poutaraud, A.; Claudel, P.; Mestre, P.; Prado, E.; Santos-Rosa, M.; Wiedemann-Merdinoglu, S.; Karst, F.; Merdinoglu, D.; Hugueney, P.
    A stress-inducible resveratrol O-methyltransferase involved in the biosynthesis of pterostilbene in grapevine (2008), Plant Physiol., 148, 1630-1639.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.240 expression in Escherichia coli. Transient coexpression of ROMT and grapevine stilbene synthase in tobacco (Nicotiana benthamiana) using the agroinfiltration technique results in the accumulation of pterostilbene in tobacco tissues Vitis vinifera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.240 0.012
-
 resveratrol pH 7.5, temperature not specified in the publication Vitis vinifera
2.1.1.240 0.014
-
 3-methoxy-4',5-dihydroxy-trans-stilbene pH 7.5, temperature not specified in the publication Vitis vinifera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.240 2 S-adenosyl-L-methionine + resveratrol Vitis vinifera the enzyme catalyses the biosynthesis of pterostilbene from resveratrol 2 S-adenosyl-L-homocysteine + pterostilbene
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.240 Vitis vinifera
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.240
-
 Vitis vinifera

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.240 2 S-adenosyl-L-methionine + trans-resveratrol = 2 S-adenosyl-L-homocysteine + pterostilbene overall reaction Vitis vinifera
a
2.1.1.240 S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene = S-adenosyl-L-homocysteine + pterostilbene (1b) Vitis vinifera
a
2.1.1.240 S-adenosyl-L-methionine + trans-resveratrol = S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene (1a) Vitis vinifera
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.240 leaf
-
 Vitis vinifera
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.240 2 S-adenosyl-L-methionine + resveratrol
-
 Vitis vinifera 2 S-adenosyl-L-homocysteine + pterostilbene
-
 ?
2.1.1.240 2 S-adenosyl-L-methionine + resveratrol the enzyme catalyses the biosynthesis of pterostilbene from resveratrol Vitis vinifera 2 S-adenosyl-L-homocysteine + pterostilbene
-
 ?
2.1.1.240 S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene resveratrol monomethyl ether i.e. 3-methoxy-4',5-dihydroxy-trans-stilbene Vitis vinifera S-adenosyl-L-homocysteine + pterostilbene pterostilbene i.e. 3,5-dimethoxy-4'-hydroxy-trans-stilbene ?
2.1.1.240 S-adenosyl-L-methionine + resveratrol
-
 Vitis vinifera S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene resveratrol monomethyl ether i.e. 3-methoxy-4',5-dihydroxy-trans-stilbene ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.240 resveratrol O-methyltransferase
-
 Vitis vinifera
2.1.1.240 ROMT
-
 Vitis vinifera

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.240 0.062
-
 resveratrol pH 7.5, temperature not specified in the publication Vitis vinifera
2.1.1.240 0.086
-
 3-methoxy-4',5-dihydroxy-trans-stilbene pH 7.5, temperature not specified in the publication Vitis vinifera

Expression

EC Number Organism Comment Expression
2.1.1.240 Vitis vinifera ROMT gene expression in grapevine leaves is induced by different stresses, including downy mildew (Plasmopara viticola) infection, ultraviolet light, and AlCl3 treatment up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.240 5.16
-
 resveratrol pH 7.5, temperature not specified in the publication Vitis vinifera
2.1.1.240 6.14
-
 3-methoxy-4',5-dihydroxy-trans-stilbene pH 7.5, temperature not specified in the publication Vitis vinifera