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Literature summary extracted from
- Cloning and expression pattern of a gene encoding an alpha-xylosidase active against xyloglucan oligosaccharides from Arabidopsis (2001), Plant Physiol., 126, 910-920.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.177 | the gene is introduced in a suitable expression vector and used to transform Saccharomyces cerevisiae | Arabidopsis thaliana |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.177 | 89000 | - |
x * 89000, SDS-PAGE | Brassica oleracea var. capitata |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.177 | Arabidopsis thaliana | Q9S7Y7 | - |
- |
| 3.2.1.177 | Brassica oleracea var. capitata | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.177 | additional information | contains eight possible glycosylation sites | Arabidopsis thaliana |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.177 | - |
Brassica oleracea var. capitata |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.177 | leaf | - |
Brassica oleracea var. capitata | - |
| 3.2.1.177 | leaf | alpha-xylosidase activity and AtXYL1 expression increase from older to younger leaves | Arabidopsis thaliana | - |
| 3.2.1.177 | seedling | apoplastic extraction | Arabidopsis thaliana | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.177 | 1.7 | - |
pH 4.5, 40°C | Brassica oleracea var. capitata |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.177 | xyloglucan + H2O | xyloglucan oligosaccharide from tamarind seed xyloglucan | Brassica oleracea var. capitata | xylose + ? | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.177 | ? | x * 89000, SDS-PAGE | Brassica oleracea var. capitata |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.177 | AtXYL1 | - |
Arabidopsis thaliana |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.177 | 4.5 | - |
- |
Brassica oleracea var. capitata |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.177 | Brassica oleracea var. capitata | isoelectric focusing | - |
8.3 |
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Release 2023.1 (January 2023)
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