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Literature summary extracted from
- Acyl-ACP thioesterases from macadamia (Macadamia tetraphylla) nuts: cloning, characterization and their impact on oil composition (2011), Plant Physiol. Biochem., 49, 82-87.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.2.14 | a His-tagged version expressed in Escherichia coli XL-1 blue | Macadamia tetraphylla |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.2.14 | 0.00008 | - |
oleoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla |  |
| 3.1.2.14 | 0.00035 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
| 3.1.2.14 | 0.00096 | - |
palmitoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
| 3.1.2.14 | 0.00297 | - |
oleoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
| 3.1.2.14 | 0.0036 | - |
palmitoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
| 3.1.2.14 | 0.00551 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
| 3.1.2.14 | 0.00563 | - |
stearoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
| 3.1.2.14 | 0.00954 | - |
stearoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.14 | 45900 | - |
x * 45900, calculated | Macadamia tetraphylla |
| 3.1.2.14 | 46200 | - |
x * 46200, calculated | Macadamia tetraphylla |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.2.14 | Macadamia tetraphylla | D2E6T0 | - |
- |
| 3.1.2.14 | Macadamia tetraphylla | D5FFZ2 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.2.14 | immobilized metal ion affinity chromatography (Ni2+) | Macadamia tetraphylla |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.2.14 | kernel | developing kernel | Macadamia tetraphylla | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.14 | 0.043 | - |
palmitoyl-[acyl-carrier protein], pH 8.0, 22°C | Macadamia tetraphylla |
| 3.1.2.14 | 0.262 | - |
palmitoleoyl-[acyl-carrier protein], pH 8.0, 22°C | Macadamia tetraphylla |
| 3.1.2.14 | 0.324 | - |
stearoyl-[acyl-carrier protein], pH 8.0, 22°C | Macadamia tetraphylla |
| 3.1.2.14 | 0.437 | - |
oleoyl-[acyl-carrier protein], pH 8.0, 22°C | Macadamia tetraphylla |
| 3.1.2.14 | 0.744 | - |
palmitoleoyl-[acyl-carrier protein], pH 8.0, 22°C | Macadamia tetraphylla |
| 3.1.2.14 | 0.778 | - |
palmitoyl-[acyl-carrier protein], pH 8.0, 22°C | Macadamia tetraphylla |
| 3.1.2.14 | 1.45 | - |
stearoyl-[acyl-carrier protein], pH 8.0, 22°C | Macadamia tetraphylla |
| 3.1.2.14 | 1.61 | - |
oleoyl-[acyl-carrier protein], pH 8.0, 22°C | Macadamia tetraphylla |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.2.14 | oleoyl-[acyl-carrier protein] + H2O | - |
Macadamia tetraphylla | acyl-carrier protein + oleic acid | - |
? | |
| 3.1.2.14 | palmitoleoyl-[acyl-carrier protein] + H2O | - |
Macadamia tetraphylla | acyl-carrier protein + palmitoleic acid | - |
? | |
| 3.1.2.14 | palmitoyl-[acyl-carrier protein] + H2O | - |
Macadamia tetraphylla | acyl-carrier protein + palmitic acid | - |
? | |
| 3.1.2.14 | stearoyl-[acyl-carrier protein] + H2O | - |
Macadamia tetraphylla | acyl-carrier protein + stearic acid | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.2.14 | ? | x * 45900, calculated | Macadamia tetraphylla |
| 3.1.2.14 | ? | x * 46200, calculated | Macadamia tetraphylla |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.2.14 | acyl-ACP thioesterase FatA type | - |
Macadamia tetraphylla |
| 3.1.2.14 | acyl-ACP thioesterase FatB type | - |
Macadamia tetraphylla |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.2.14 | 0.03 | - |
palmitoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
| 3.1.2.14 | 0.15 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
| 3.1.2.14 | 0.18 | - |
stearoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
| 3.1.2.14 | 0.25 | - |
oleoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
| 3.1.2.14 | 0.44 | - |
palmitoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
| 3.1.2.14 | 0.45 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
| 3.1.2.14 | 0.88 | - |
stearoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
| 3.1.2.14 | 0.98 | - |
oleoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.2.14 | Macadamia tetraphylla | calculated | - |
6.2 |
| 3.1.2.14 | Macadamia tetraphylla | calculated | - |
7.1 |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.2.14 | 30 | - |
palmitoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
| 3.1.2.14 | 30 | - |
stearoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
| 3.1.2.14 | 30 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
| 3.1.2.14 | 80 | - |
oleoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
| 3.1.2.14 | 90 | - |
stearoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
| 3.1.2.14 | 120 | - |
palmitoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
| 3.1.2.14 | 1300 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
| 3.1.2.14 | 12910 | - |
oleoyl-[acyl-carrier protein] | pH 8.0, 22°C | Macadamia tetraphylla | |
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