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Literature summary extracted from
- Arabidopsis nucleoside hydrolases involved in intracellular and extracellular degradation of purines (2011), Plant J., 65, 703-711.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.2.1 | 0.0431 | - |
adenosine | at 30°C, pH not specified in the publication | Arabidopsis thaliana |  |
| 3.2.2.1 | 0.0972 | - |
Inosine | at 30°C, pH not specified in the publication | Arabidopsis thaliana | |
| 3.2.2.2 | 0.44 | - |
methyluridine | pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 3.2.2.2 | 0.7 | - |
adenosine | pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 3.2.2.2 | 0.8 | - |
uridine | pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 3.2.2.2 | 1.4 | - |
Inosine | pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 3.2.2.2 | 1.69 | - |
Xanthosine | pH and temperature not specified in the publication | Arabidopsis thaliana | |
| 3.2.2.3 | 0.44 | - |
5-methyluridine | at 30°C, pH not specified in the publication | Arabidopsis thaliana | |
| 3.2.2.3 | 0.7 | - |
adenosine | at 30°C, pH not specified in the publication | Arabidopsis thaliana | |
| 3.2.2.3 | 0.8 | - |
uridine | at 30°C, pH not specified in the publication | Arabidopsis thaliana | |
| 3.2.2.3 | 1.4 | - |
Inosine | at 30°C, pH not specified in the publication | Arabidopsis thaliana | |
| 3.2.2.3 | 1.69 | - |
Xanthosine | at 30°C, pH not specified in the publication | Arabidopsis thaliana | |
| 3.2.2.8 | 0.44 | - |
methyluridine | isoform NSH1, pH not specified in the publication, at 30°C | Arabidopsis thaliana | |
| 3.2.2.8 | 0.7 | - |
adenosine | isoform NSH1, pH not specified in the publication, at 30°C | Arabidopsis thaliana | |
| 3.2.2.8 | 0.8 | - |
uridine | isoform NSH1, pH not specified in the publication, at 30°C | Arabidopsis thaliana | |
| 3.2.2.8 | 1.4 | - |
Inosine | isoform NSH1, pH not specified in the publication, at 30°C | Arabidopsis thaliana | |
| 3.2.2.8 | 1.69 | - |
Xanthosine | isoform NSH1, pH not specified in the publication, at 30°C | Arabidopsis thaliana | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.2.1 | extracellular | - |
Arabidopsis thaliana | - |
- |
| 3.2.2.2 | cytosol | - |
Arabidopsis thaliana | 5829 | - |
| 3.2.2.8 | cytosol | isoform NSH2 | Arabidopsis thaliana | 5829 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.2.1 | Arabidopsis thaliana | - |
ecotype Columbia | - |
| 3.2.2.2 | Arabidopsis thaliana | - |
ecotype Columbia | - |
| 3.2.2.3 | Arabidopsis thaliana | - |
ecotype Columbia | - |
| 3.2.2.8 | Arabidopsis thaliana | - |
ecotype Columbia | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.2.1 | leaf | - |
Arabidopsis thaliana | - |
| 3.2.2.2 | leaf | - |
Arabidopsis thaliana | - |
| 3.2.2.3 | leaf | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.2.1 | adenosine + H2O | - |
Arabidopsis thaliana | D-ribose + adenine | - |
? | |
| 3.2.2.1 | inosine + H2O | - |
Arabidopsis thaliana | D-ribose + hypoxanthine | - |
? | |
| 3.2.2.2 | 5-methyluridine + H2O | - |
Arabidopsis thaliana | D-ribose + 5-methyluracil | - |
? | |
| 3.2.2.2 | adenosine + H2O | - |
Arabidopsis thaliana | D-ribose + adenine | - |
? | |
| 3.2.2.2 | inosine + H2O | isoform NSH2 shows highest hydrolysis capacity for inosine | Arabidopsis thaliana | D-ribose + hypoxanthine | - |
? | |
| 3.2.2.2 | uridine + H2O | - |
Arabidopsis thaliana | D-ribose + uracil | - |
? | |
| 3.2.2.2 | xanthosine + H2O | - |
Arabidopsis thaliana | D-ribose + xanthine | - |
? | |
| 3.2.2.3 | 5-methyluridine + H2O | - |
Arabidopsis thaliana | D-ribose + 5-methyluracil | - |
? | |
| 3.2.2.3 | adenosine + H2O | - |
Arabidopsis thaliana | D-ribose + adenine | - |
? | |
| 3.2.2.3 | inosine + H2O | - |
Arabidopsis thaliana | D-ribose + hypoxanthine | - |
? | |
| 3.2.2.3 | isopentenyladenine riboside + H2O | - |
Arabidopsis thaliana | D-ribose + N6-isopentenyladenine | - |
? | |
| 3.2.2.3 | uridine + H2O | isoform NSH1 shows highest hydrolysis capacity for uridine | Arabidopsis thaliana | D-ribose + uracil | - |
? | |
| 3.2.2.3 | xanthosine + H2O | - |
Arabidopsis thaliana | D-ribose + xanthine | - |
? | |
| 3.2.2.8 | 5-methyluridine + H2O | - |
Arabidopsis thaliana | D-ribose + 5-methyluracil | - |
? | |
| 3.2.2.8 | adenosine + H2O | - |
Arabidopsis thaliana | adenine + D-ribose | - |
? | |
| 3.2.2.8 | inosine + H2O | - |
Arabidopsis thaliana | hypoxanthine + D-ribose | - |
? | |
| 3.2.2.8 | isopentenyladenine riboside + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
| 3.2.2.8 | uridine + H2O | - |
Arabidopsis thaliana | uracil + D-ribose | - |
? | |
| 3.2.2.8 | xanthosine + H2O | high hydrolytic efficiency, xanthosine is a better substrate for isoform NSH1 compared to inosine and adenosine | Arabidopsis thaliana | xanthine + D-ribose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.2.1 | NSH3 | isoform | Arabidopsis thaliana |
| 3.2.2.1 | purine hydrolase | - |
Arabidopsis thaliana |
| 3.2.2.2 | NSH2 | isoform | Arabidopsis thaliana |
| 3.2.2.3 | NSH1 | isoform, former designation URH1 | Arabidopsis thaliana |
| 3.2.2.8 | NSH1 | former designation URH1 | Arabidopsis thaliana |
| 3.2.2.8 | nucleoside hydrolase | - |
Arabidopsis thaliana |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.2.2.1 | physiological function | isoform NSH3 functions as an extracellular, purine-specific hydrolase that is involved in degradation of extracellular nucleosides and participates in wound and pathogen responses | Arabidopsis thaliana |
| 3.2.2.2 | physiological function | isoform NSH2 acts during the late phase of senescence and supports inosine breakdown | Arabidopsis thaliana |
| 3.2.2.3 | malfunction | a knockout mutant for isoform NSH1 shows symptoms of accelerated senescence, accompanied by marked accumulation of uridine and xanthosine under conditions of prolonged darkness | Arabidopsis thaliana |
| 3.2.2.3 | physiological function | isoform NSH1 represents the leading activity in purine and pyrimidine breakdown in a cell | Arabidopsis thaliana |
| 3.2.2.8 | malfunction | a knockout mutant for NSH1 shows symptoms of accelerated senescence, accompanied by marked accumulation of uridine and xanthosine under conditions of prolonged darkness. | Arabidopsis thaliana |
| 3.2.2.8 | physiological function | isoform NSH1 represents the leading activity for both pyrimidine and purine nucleoside hydrolysis. Isoform NSH3 functions as an extracellular, purine-specific hydrolase that is involved in degradation of extracellular nucleosides and may participate in wound and pathogen responses. Isoform NSH2 acts during the late phase of senescence and may support inosine breakdown | Arabidopsis thaliana |
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