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Literature summary extracted from

  • Seki, H.; Sawai, S.; Ohyama, K.; Mizutani, M.; Ohnishi, T.; Sudo, H.; Fukushima, E.O.; Akashi, T.; Aoki, T.; Saito, K.; Muranaka, T.
    Triterpene functional genomics in Licorice for identification of CYP72A154 involved in the biosynthesis of glycyrrhizin (2011), Plant Cell, 23, 4112-4123.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.115 expressed in Escherichia coli DH10Bac cells and in Saccharomyces cerevisiae strain BJ2168 Glycyrrhiza uralensis
1.14.14.115 expressed in Saccharomyces cerevisiae strain BJ2168 that endogenously produces 11-oxo-beta-amyrin Glycyrrhiza uralensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.115 11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase] Glycyrrhiza uralensis the enzyme mainly catalyzes the C-30 hydroxylation of 11-oxo-beta-amyrin to yield 30-hydroxy-11-oxo-beta-amyrin as the major product, accompanied by its isomers as minor products 30-hydroxy-11-oxo-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.115 Glycyrrhiza uralensis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.115 colon
-
 Glycyrrhiza uralensis
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1.14.14.115 additional information not detected in leaves Glycyrrhiza uralensis
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1.14.14.115 root
-
 Glycyrrhiza uralensis
-
1.14.14.115 stem
-
 Glycyrrhiza uralensis
-
1.14.14.115 stolon
-
 Glycyrrhiza uralensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.115 11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase] single oxidation reaction catalyzed by CYP72A154 Glycyrrhiza uralensis 30-hydroxy-11-oxo-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.115 11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase] the enzyme mainly catalyzes the C-30 hydroxylation of 11-oxo-beta-amyrin to yield 30-hydroxy-11-oxo-beta-amyrin as the major product, accompanied by its isomers as minor products Glycyrrhiza uralensis 30-hydroxy-11-oxo-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.115 30-hydroxy-11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase]
-
 Glycyrrhiza uralensis glycyrrhetaldehyde + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.14.115 30-hydroxy-11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase] single oxidation reaction catalyzed by CYP72A154 Glycyrrhiza uralensis glycyrrhetaldehyde + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.14.115 glycyrrhetaldehyde + O2 + [reduced NADPH-hemoprotein reductase]
-
 Glycyrrhiza uralensis glycyrrhetinate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.115 CYP72A154
-
 Glycyrrhiza uralensis
1.14.14.115 CYP88D6
-
 Glycyrrhiza uralensis

General Information

EC Number General Information Comment Organism
1.14.14.115 metabolism the beta-amyrin 11-oxidase gene CYP88D6 is the initial P450 gene in glycyrrhizin biosynthesis. CYP72A154 is responsible for C-30 oxidation of beta-amyrin in the glycyrrhizin pathway Glycyrrhiza uralensis
1.14.14.115 metabolism the enzyme is involved in the biosynthesis of glycyrrhizin Glycyrrhiza uralensis