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Literature summary extracted from

  • Yun, M.S.; Umemoto, T.; Kawagoe, Y.
    Rice debranching enzyme isoamylase3 facilitates starch metabolism and affects plastid morphogenesis (2011), Plant Cell Physiol., 52, 1068-1082.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.68 expressed in Escherichia coli BL21(DE3) cells Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.68 plastid stroma
-
Oryza sativa 9532
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.68 79000
-
x * 79000, SDS-PAGE Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.68 Oryza sativa
-
cultivar Nipponbare
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.68 endosperm
-
Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.68 amylopectin + H2O
-
Oryza sativa ?
-
?
3.2.1.68 beta-limit dextrin + H2O
-
Oryza sativa ?
-
?
3.2.1.68 additional information recombinant isoamylase 3 does not readily hydrolyze red pullulan and amylose Oryza sativa ?
-
?
3.2.1.68 starch + H2O
-
Oryza sativa ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.68 ? x * 79000, SDS-PAGE Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
3.2.1.68 ISA3
-
Oryza sativa

General Information

EC Number General Information Comment Organism
3.2.1.68 malfunction both overexpression and loss of function of isoamylase 3 in the endosperm generated pleomorphic amyloplasts and starch granules Oryza sativa
3.2.1.68 physiological function isoamylase 3 facilitates starch metabolism and affects morphological characteristics of plastids in rice. Both overexpression and loss of function of isoamylase 3 in the endosperm generates pleomorphic amyloplasts and starch granules Oryza sativa