BRENDA - Enzyme Database

Molecular cloning and functional characterization of Schistosoma japonicum enolase which is highly expressed at the schistosomulum stage

Yang, J.; Qiu, C.; Xia, Y.; Yao, L.; Fu, Z.; Yuan, C.; Feng, X.; Lin, J.; Parasitol. Res. 107, 667-677 (2010)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
4.2.1.11
medicine
the recombinant enzyme protein is a useful antigen in vaccination against adult worms
Schistosoma japonicum
Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli
Schistosoma japonicum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
additional information
-
additional information
kinetic analysis, forward and reverse reaction, overview
Schistosoma japonicum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
activates
Schistosoma japonicum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
50000
-
x * 50000, recombinant His-tagged enolase, SDS-PAGE
Schistosoma japonicum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Schistosoma japonicum
-
phosphoenolpyruvate + H2O
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Schistosoma japonicum
Q5DDV5
Anhui isolate, Chinese mainland strain, propagated in BALB/c mice
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
recombinant soluble His-tagged enzyme from Escherichia coli by nickel affinity chromatography
Schistosoma japonicum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
additional information
the enolase gene is highly expressed at 18-28 days of the life cycle, RT-PCR expression analysis
Schistosoma japonicum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
15.87
-
purified recombinant enzyme, substrate phosphoenolpyruvate, pH 7.4, 20°C
Schistosoma japonicum
4.2.1.11
35.81
-
purified recombinant enzyme, substrate 2-phospho-D-glycerate, pH 7.4, 20°C
Schistosoma japonicum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
716427
Schistosoma japonicum
phosphoenolpyruvate + H2O
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 50000, recombinant His-tagged enolase, SDS-PAGE
Schistosoma japonicum
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.11
20
-
assay at
Schistosoma japonicum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6.5
7
-
Schistosoma japonicum
Application (protein specific)
EC Number
Application
Commentary
Organism
4.2.1.11
medicine
the recombinant enzyme protein is a useful antigen in vaccination against adult worms
Schistosoma japonicum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli
Schistosoma japonicum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
additional information
-
additional information
kinetic analysis, forward and reverse reaction, overview
Schistosoma japonicum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
activates
Schistosoma japonicum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
50000
-
x * 50000, recombinant His-tagged enolase, SDS-PAGE
Schistosoma japonicum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Schistosoma japonicum
-
phosphoenolpyruvate + H2O
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
recombinant soluble His-tagged enzyme from Escherichia coli by nickel affinity chromatography
Schistosoma japonicum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
additional information
the enolase gene is highly expressed at 18-28 days of the life cycle, RT-PCR expression analysis
Schistosoma japonicum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
15.87
-
purified recombinant enzyme, substrate phosphoenolpyruvate, pH 7.4, 20°C
Schistosoma japonicum
4.2.1.11
35.81
-
purified recombinant enzyme, substrate 2-phospho-D-glycerate, pH 7.4, 20°C
Schistosoma japonicum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
716427
Schistosoma japonicum
phosphoenolpyruvate + H2O
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 50000, recombinant His-tagged enolase, SDS-PAGE
Schistosoma japonicum
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.11
20
-
assay at
Schistosoma japonicum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6.5
7
-
Schistosoma japonicum
General Information
EC Number
General Information
Commentary
Organism
4.2.1.11
metabolism
enolase is a key enzyme in the glycolytic pathway
Schistosoma japonicum
4.2.1.11
additional information
the recombinant enolase is recognized by rabbit sera directed against an antigen preparation from adult worms. 24.28% reduction in the liver egg count and a reduction of 21.45% in the fecal egg count occur in BALB/c mice vaccinated with recombinant SjENO compared with control mice
Schistosoma japonicum
4.2.1.11
physiological function
recombinant SjENO binds to human plasminogen as its receptor
Schistosoma japonicum
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.2.1.11
metabolism
enolase is a key enzyme in the glycolytic pathway
Schistosoma japonicum
4.2.1.11
additional information
the recombinant enolase is recognized by rabbit sera directed against an antigen preparation from adult worms. 24.28% reduction in the liver egg count and a reduction of 21.45% in the fecal egg count occur in BALB/c mice vaccinated with recombinant SjENO compared with control mice
Schistosoma japonicum
4.2.1.11
physiological function
recombinant SjENO binds to human plasminogen as its receptor
Schistosoma japonicum