Any feedback?
Literature summary extracted from
- Molecular cloning and functional characterization of Schistosoma japonicum enolase which is highly expressed at the schistosomulum stage (2010), Parasitol. Res., 107, 667-677.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 4.2.1.11 | medicine | the recombinant enzyme protein is a useful antigen in vaccination against adult worms | Schistosoma japonicum |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.11 | DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli | Schistosoma japonicum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.11 | additional information | - |
additional information | kinetic analysis, forward and reverse reaction, overview | Schistosoma japonicum |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.11 | Mg2+ | activates | Schistosoma japonicum |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.11 | 50000 | - |
x * 50000, recombinant His-tagged enolase, SDS-PAGE | Schistosoma japonicum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.11 | 2-phospho-D-glycerate | Schistosoma japonicum | - |
phosphoenolpyruvate + H2O | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.11 | Schistosoma japonicum | Q5DDV5 | Anhui isolate, Chinese mainland strain, propagated in BALB/c mice | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.11 | recombinant soluble His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Schistosoma japonicum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.2.1.11 | additional information | the enolase gene is highly expressed at 18-28 days of the life cycle, RT-PCR expression analysis | Schistosoma japonicum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.11 | 15.87 | - |
purified recombinant enzyme, substrate phosphoenolpyruvate, pH 7.4, 20°C | Schistosoma japonicum |
| 4.2.1.11 | 35.81 | - |
purified recombinant enzyme, substrate 2-phospho-D-glycerate, pH 7.4, 20°C | Schistosoma japonicum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.11 | 2-phospho-D-glycerate | - |
Schistosoma japonicum | phosphoenolpyruvate + H2O | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.11 | ? | x * 50000, recombinant His-tagged enolase, SDS-PAGE | Schistosoma japonicum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.11 | enolase | - |
Schistosoma japonicum |
| 4.2.1.11 | SjENO | - |
Schistosoma japonicum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.11 | 20 | - |
assay at | Schistosoma japonicum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.11 | 6.5 | 7 | - |
Schistosoma japonicum |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.2.1.11 | metabolism | enolase is a key enzyme in the glycolytic pathway | Schistosoma japonicum |
| 4.2.1.11 | additional information | the recombinant enolase is recognized by rabbit sera directed against an antigen preparation from adult worms. 24.28% reduction in the liver egg count and a reduction of 21.45% in the fecal egg count occur in BALB/c mice vaccinated with recombinant SjENO compared with control mice | Schistosoma japonicum |
| 4.2.1.11 | physiological function | recombinant SjENO binds to human plasminogen as its receptor | Schistosoma japonicum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
