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Literature summary extracted from

  • Sharma, S.K.; Banyal, H.S.
    Characterization of Plasmodium berghei glutathione synthetase (2011), Parasitol. Int., 60, 321-323.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.3 Al3+ 40% residual activity Plasmodium berghei
6.3.2.3 Ba2+ 27% residual activity Plasmodium berghei
6.3.2.3 Cd2+ complete inhibition Plasmodium berghei
6.3.2.3 Chloroquine 0.1 mM, 24% inhibition Plasmodium berghei
6.3.2.3 Co2+ complete inhibition Plasmodium berghei
6.3.2.3 Cu2+ 15% residual activity Plasmodium berghei
6.3.2.3 Fe3+ 24% residual activity Plasmodium berghei
6.3.2.3 glutathione noncompetitive Plasmodium berghei
6.3.2.3 Hg2+ complete inhibition Plasmodium berghei
6.3.2.3 mefloquine 0.1 mM, 21% inhibition Plasmodium berghei
6.3.2.3 additional information no significant inhibition: amoxycillin, gentamycin, streptomycin, kanamycin, tetracycline, penicillin, artesunate, aablaquine and primaquine Plasmodium berghei
6.3.2.3 Ni2+ complete inhibition Plasmodium berghei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.3 0.33
-
 ATP pH 8.2, temperature not specified in the publication Plasmodium berghei
6.3.2.3 0.43
-
 Gly pH 8.2, 37°C Plasmodium berghei
6.3.2.3 8.3
-
 gamma-L-Glu-L-Cys pH 8.2, 37°C Plasmodium berghei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.3 70000
-
 gel filtration Plasmodium berghei

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.3 Plasmodium berghei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.3 from Plasmodium berghei infected mouse erythrocytes Plasmodium berghei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.3 29.95
-
 pH 8.2, 37°C Plasmodium berghei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.3 ATP + gamma-L-Glu-L-Cys + Gly
-
 Plasmodium berghei ADP + phosphate + glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.3 monomer x * 70000, SDS-PAGE Plasmodium berghei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.3 37
-
-
 Plasmodium berghei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.2.3 60
-
 10 min, no residual activity Plasmodium berghei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.3 8 8.5
-
 Plasmodium berghei