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Literature summary extracted from

  • Kharel, M.K.; Lian, H.; Rohr, J.
    Characterization of the TDP-D-ravidosamine biosynthetic pathway: one-pot enzymatic synthesis of TDP-D-ravidosamine from thymidine-5-phosphate and glucose-1-phosphate (2011), Org. Biomol. Chem., 9, 1799-1808.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.1.1.236 synthesis synthesis of TDP-D-ravidosamine, necessary for future in vitro glycosylation assays. TDP-D-ravidosamine is the anticipated sugar donor substrate of RavGT (the glycosyltransferase that links D-ravidosamine to the polyketide derived backbone defuco-gilvocarcin V). Defuco-gilvocarcin V exhibits superior anticancer/antibacterial activities Streptomyces ravidus
2.6.1.90 synthesis synthesis of TDP-D-ravidosamine, necessary for future in vitro glycosylation assays. TDP-D-ravidosamine is the anticipated sugar donor substrate of RavGT (the glycosyltransferase that links D-ravidosamine to the polyketide derived backbone defuco-gilvocarcin V). Defuco-gilvocarcin V exhibits superior anticancer/antibacterial activities Streptomyces ravidus
5.3.2.3 synthesis synthesis of TDP-D-ravidosamine, necessary for in vitro glycosylation assays. TDP-D-ravidosamine is the anticipated sugar donor substrate of RavGT (the glycosyltransferase that links D-ravidosamine to the polyketide derived backbone defuco-gilvocarcin V). Defuco-gilvocarcin V exhibits superior anticancer/antibacterial activities Streptomyces ravidus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.236 expression in Escherichia coli Streptomyces ravidus
2.6.1.90 expression in Escherichia coli Streptomyces ravidus
5.3.2.3 expression in Escherichia coli Streptomyces ravidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.236 31060
-
 2 * 31060, SDS-PAGE Streptomyces ravidus
2.6.1.90 41710
-
 2 * 41710, SDS-PAGE Streptomyces ravidus
5.3.2.3 19100
-
 2 * 19100, SDS-PAGE Streptomyces ravidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.90 dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose + L-glutamate Streptomyces ravidus the enzyme is involved in the conversion of D-glucose-1-phosphate to dTDP-D-ravidosamine dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + 2-oxoglutarate
-
 ?
5.3.2.3 dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose Streptomyces ravidus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.236 Streptomyces ravidus
-
-
-
2.6.1.90 Streptomyces ravidus
-
-
-
5.3.2.3 Streptomyces ravidus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.236
-
 Streptomyces ravidus
2.6.1.90
-
 Streptomyces ravidus
5.3.2.3
-
 Streptomyces ravidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.236 2 S-adenosyl-L-methionine + dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose RavNMT catalyzes the transfer of methyl groups from S-adenosyl-L-methionine to the amino nitrogen of in a sequential manner Streptomyces ravidus 2 S-adenosyl-L-homocysteine + TDP-3-dimethylamino-3,6-dideoxy-alpha-D-galactopyranose TDP-3-dimethylamino-3,6-dideoxy-alpha-D-galactopyranose i.e. TDP-D-ravidosamine ?
2.6.1.90 dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose + L-glutamate
-
 Streptomyces ravidus dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + 2-oxoglutarate
-
 ?
2.6.1.90 dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose + L-glutamate the enzyme is involved in the conversion of D-glucose-1-phosphate to dTDP-D-ravidosamine Streptomyces ravidus dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + 2-oxoglutarate
-
 ?
5.3.2.3 dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose
-
 Streptomyces ravidus ?
-
 ?
5.3.2.3 dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose
-
 Streptomyces ravidus ? no characterization of the product due to its low yield ?

Subunits

EC Number Subunits Comment Organism
2.1.1.236 dimer 2 * 31060, SDS-PAGE Streptomyces ravidus
2.6.1.90 dimer 2 * 41710, SDS-PAGE Streptomyces ravidus
5.3.2.3 dimer 2 * 19100, SDS-PAGE Streptomyces ravidus

Synonyms

EC Number Synonyms Comment Organism
2.1.1.236 RavNMT
-
 Streptomyces ravidus
2.6.1.90 RavAMT
-
 Streptomyces ravidus
2.6.1.90 TDP-3-keto-6-deoxy-D-galactose-3-aminotransferase
-
 Streptomyces ravidus
5.3.2.3 RavIM
-
 Streptomyces ravidus
5.3.2.3 TDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase
-
 Streptomyces ravidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.236 37
-
 assay at Streptomyces ravidus
2.6.1.90 37
-
 assay at Streptomyces ravidus
2.6.1.90 50 55
-
 Streptomyces ravidus
5.3.2.3 37
-
 assay at Streptomyces ravidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.236 7.5
-
 assay at Streptomyces ravidus
2.6.1.90 7.5
-
 assay at Streptomyces ravidus
5.3.2.3 7.5
-
 assay at Streptomyces ravidus