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Literature summary extracted from

  • Su, D.; Lieberman, A.; Lang, B.F.; Simonovic, M.; Soell, D.; Ling, J.
    An unusual tRNAThr derived from tRNAHis reassigns in yeast mitochondria the CUN codons to threonine (2011), Nucleic Acids Res., 39, 4866-4874.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.21 HTS1, phylogenetic analysis, expression in Escherichia coli strain BL21(DE3) Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.3 additional information
-
 additional information kinetics of MST1 with different tRNAs, overview Saccharomyces cerevisiae
6.1.1.3 0.00029
-
 tRNAThr pH 7.2, 37°C, tRNAThr1, MST1 Saccharomyces cerevisiae
6.1.1.3 0.00044
-
 tRNAThr pH 7.2, 37°C, tRNAThr2, MST1 Saccharomyces cerevisiae
6.1.1.3 0.0015
-
 tRNAThr pH 7.2, 37°C, tRNAThr1(G-1), MST1 Saccharomyces cerevisiae
6.1.1.21 0.00043
-
 tRNAHis pH 7.2, 37°C, wild-type substrate Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.21 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.3 Mg2+
-
 Saccharomyces cerevisiae
6.1.1.3 Mg2+
-
 Candida albicans
6.1.1.21 Mg2+ required Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.3 ATP + L-threonine + tRNAThr Saccharomyces cerevisiae
-
 AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
6.1.1.3 ATP + L-threonine + tRNAThr Candida albicans
-
 AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
6.1.1.21 ATP + L-histidine + tRNAHis Saccharomyces cerevisiae
-
 AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.3 Candida albicans
-
-
-
6.1.1.3 Saccharomyces cerevisiae
-
-
-
6.1.1.21 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.3 ATP + L-threonine + tRNAThr
-
 Saccharomyces cerevisiae AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
6.1.1.3 ATP + L-threonine + tRNAThr
-
 Candida albicans AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
6.1.1.3 ATP + L-threonine + tRNAThr MST1 can attach threonine to both tRNAThr1 and the regular tRNAThr2, but not to the wild-type tRNAHis. But a loss of the first nucleotide G-1 in tRNAHis converts it to a substrate for MST1 Saccharomyces cerevisiae AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
6.1.1.3 additional information addition of first nucleotide G-1 to tRNAThr1 allows efficient histidylation by histidyl-tRNA synthetase Saccharomyces cerevisiae ?
-
 ?
6.1.1.21 ATP + L-histidine + tRNAHis
-
 Saccharomyces cerevisiae AMP + diphosphate + L-histidyl-tRNAHis
-
 ?
6.1.1.21 ATP + L-histidine + tRNAHis active with wild-type and mutant C73A and A37 insertion tRNAHis Saccharomyces cerevisiae AMP + diphosphate + L-histidyl-tRNAHis
-
 ?
6.1.1.21 additional information addition of a first nucleotide to tRNAThr1 allows efficient histidylation by histidyl-tRNA synthetase. Loss of the first nucleotide of wild-type tRNAHis converts it to a substrate for threonyl-tRNA sythetase, EC 6.1.1.3 Saccharomyces cerevisiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.3 mitochondrial threonyl-tRNA synthetase
-
 Saccharomyces cerevisiae
6.1.1.3 mitochondrial threonyl-tRNA synthetase
-
 Candida albicans
6.1.1.3 Mst1
-
 Saccharomyces cerevisiae
6.1.1.3 Mst1
-
 Candida albicans
6.1.1.21 Histidyl-tRNA synthetase
-
 Saccharomyces cerevisiae
6.1.1.21 HTS1
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.3 37
-
 assay at Saccharomyces cerevisiae
6.1.1.3 37
-
 assay at Candida albicans
6.1.1.21 37
-
 assay at Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.3 0.00157
-
 tRNAThr pH 7.2, 37°C, tRNAThr1(G-1), MST1 Saccharomyces cerevisiae
6.1.1.3 0.0383
-
 tRNAThr pH 7.2, 37°C, tRNAThr2, MST1 Saccharomyces cerevisiae
6.1.1.3 0.0467
-
 tRNAThr pH 7.2, 37°C, tRNAThr1, MST1 Saccharomyces cerevisiae
6.1.1.21 0.417
-
 tRNAHis pH 7.2, 37°C, wild-type substrate Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.3 7.2
-
 assay at Saccharomyces cerevisiae
6.1.1.3 7.2
-
 assay at Candida albicans
6.1.1.21 7.2
-
 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.3 ATP
-
 Saccharomyces cerevisiae
6.1.1.3 ATP
-
 Candida albicans
6.1.1.21 ATP
-
 Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
6.1.1.3 evolution evolutionary development of unusual tRNAThr1, containing an enlarged 8-nt anticodon loop, which is required to reassign CUN codons from leucine to threonine, tRNAThr phylogenetic analysis, overview. MST1 has co-evolved with tRNAThr, which evoled from tRNAHis Saccharomyces cerevisiae
6.1.1.3 evolution tRNAThr phylogenetic analysis, overview Candida albicans
6.1.1.3 metabolism in certain yeast mitochondria, CUN codons are reassigned from leucine to threonine, which requires an unusual tRNAThr with an enlarged 8-nt anticodon loop, tRNAThr1 Saccharomyces cerevisiae
6.1.1.3 metabolism in certain yeast mitochondria, CUN codons are reassigned from leucine to threonine, which requires an unusual tRNAThr with an enlarged 8-nt anticodon loop, tRNAThr1. But in Candida albicans, the CUN codons remain assigned to leucine, and MST1 cannot threonylate tRNAThr Candida albicans
6.1.1.21 evolution phylogenetic analysis reveals that tRNAThr1 originated from tRNAHis Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.3 1.093
-
 tRNAThr pH 7.2, 37°C, tRNAThr1(G-1), MST1 Saccharomyces cerevisiae
6.1.1.3 90
-
 tRNAThr pH 7.2, 37°C, tRNAThr2, MST1 Saccharomyces cerevisiae
6.1.1.3 171.7
-
 tRNAThr pH 7.2, 37°C, tRNAThr1, MST1 Saccharomyces cerevisiae
6.1.1.21 1000
-
 tRNAHis pH 7.2, 37°C, wild-type substrate Saccharomyces cerevisiae