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Literature summary extracted from

  • Yuan, J.; Gogakos, T.; Babina, A.M.; Soell, D.; Randau, L.
    Change of tRNA identity leads to a divergent orthogonal histidyl-tRNA synthetase/tRNAHis pair (2011), Nucleic Acids Res., 39, 2286-2293.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.21 recombinant HisRS allows complete histidylation of a Caulobacter crescentus tRNAHis transcript lacking G-1, addition of G-1 does not improve the aminoacylation Caulobacter vibrioides

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.21 KCl
-
 Caulobacter vibrioides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.21 additional information
-
 additional information kinetics with diverse tRNAHIs variants, overview Caulobacter vibrioides
6.1.1.21 0.00325
-
 tRNAHis wild-type Caulobacter crescentus tRNAHis, pH 7.5, 30°C Caulobacter vibrioides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.21 Mg2+ required Caulobacter vibrioides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.21 52300
-
 x * 52300 Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.21 ATP + L-histidine + tRNAHis Caulobacter vibrioides
-
 AMP + diphosphate + L-histidyl-tRNAHis
-
 ?
6.1.1.21 additional information Caulobacter vibrioides Caulobacter crescentus HisRS shows very low activity with Escherichia coli tRNAHis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.21 Caulobacter vibrioides
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.21 ATP + L-histidine + tRNAHis
-
 Caulobacter vibrioides AMP + diphosphate + L-histidyl-tRNAHis
-
 ?
6.1.1.21 additional information Caulobacter crescentus HisRS shows very low activity with Escherichia coli tRNAHis Caulobacter vibrioides ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.21 ? x * 52300 Caulobacter vibrioides

Synonyms

EC Number Synonyms Comment Organism
6.1.1.21 HisRS
-
 Caulobacter vibrioides
6.1.1.21 Histidyl-tRNA synthetase
-
 Caulobacter vibrioides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.21 30
-
-
 Caulobacter vibrioides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.21 4
-
 tRNAHis wild-type Caulobacter crescentus tRNAHis, pH 7.5, 30°C Caulobacter vibrioides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.21 7.5
-
-
 Caulobacter vibrioides

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.21 ATP
-
 Caulobacter vibrioides

General Information

EC Number General Information Comment Organism
6.1.1.21 evolution a conserved tRNA recognition pattern change during evolution leading to a divergent orthogonal HisRS/tRNAHis pair Caulobacter vibrioides
6.1.1.21 malfunction mutations in the tRNAHis anticodon cause a drastic loss of in vitro histidylation, and mutations of bases A73 and U72 also reduce charging Caulobacter vibrioides
6.1.1.21 physiological function mature tRNAHis has at its 5'-terminus an extra guanylate, designated as G-1. This is the major recognition element for HisRS to permit acylation of tRNAHis with histidine. However, recombinant HisRS allows complete histidylation of a Caulobacter crescentus tRNAHis transcript lacking G-1, and addition of G-1 does not improve the aminoacylation, but increases Km. The major recognition elements in Caulobacter crescentus tRNAHis are the anticodon, the discriminator base and U72, which are recognized by the divergent, based on sequence similarity, Caulobacter crescentus HisRS, a divergent orthogonal HisRS/tRNAHis pair Caulobacter vibrioides

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.21 1231
-
 tRNAHis wild-type Caulobacter crescentus tRNAHis, pH 7.5, 30°C Caulobacter vibrioides