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Literature summary extracted from

  • Obado, S.O.; Bot, C.; Echeverry, M.C.; Bayona, J.C.; Alvarez, V.E.; Taylor, M.C.; Kelly, J.M.
    Centromere-associated topoisomerase activity in bloodstream form Trypanosoma brucei (2011), Nucleic Acids Res., 39, 1023-1033.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.2.2 expression of C-terminally c-Myc-tagged Topo-IIalpha Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
5.6.2.2 additional information the Topo-II decatenation reaction is completely abolished in Topo-IIalpha knockout cells, while this is not the case in Topo-IIbeta knockout cells. Expression of Topo-II can rescue the bloodstream form from the effects of Topo-IIalpha knockdown. Complementation of the Topo-IIalpha RNAi phenotype by expression of truncated versions of the Trypanosoma cruzi enzyme, overview Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.2 etoposide mediates inhibition of the DNA ligation step in the Topo-II decatenation reaction Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.2.2 centromer associated Trypanosoma brucei
-
-
5.6.2.2 additional information the enzyme contains a nuclear targeting sequence of 6 residues Trypanosoma brucei
-
-

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.2 Trypanosoma brucei
-
 clone 221a
-
5.6.2.2 Trypanosoma brucei Lister 427
-
 clone 221a
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
5.6.2.2 additional information the enzyme contains two putative sumoylation motifs, both at corresponding sites in the C-terminal region. Sumoylation of the enzyme is not required for centromere-specific cleavage activity, in contrast to other species Trypanosoma brucei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.2.2 epimastigote
-
 Trypanosoma brucei
-
5.6.2.2 additional information bloodstream form Trypanosoma brucei
-

Subunits

EC Number Subunits Comment Organism
5.6.2.2 More the variable C-terminal region of topoisomerase-II has a major role in regulating biological function Trypanosoma brucei

Synonyms

EC Number Synonyms Comment Organism
5.6.2.2 topoisomerase-II
-
 Trypanosoma brucei

General Information

EC Number General Information Comment Organism
5.6.2.2 malfunction in bloodstream form Trypanosoma brucei, RNAi-mediated depletion of topoisomerase-IIalpha, but not topoisomerase-IIbeta, results in the abolition of centromere-localized activity and is lethal Trypanosoma brucei
5.6.2.2 additional information the variable carboxyl terminal region of topoisomerase-II has a major role in regulating biological function Trypanosoma brucei
5.6.2.2 physiological function topoisomerase-II is important for chromosome segregation and accumulates at centromeres during prometaphase, where it resolves the DNA catenations that represent the last link between sister chromatids Trypanosoma brucei