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Literature summary extracted from

  • Tang, X.; Zhang, Y.; Tucker, L.; Ramratnam, B.
    Phosphorylation of the RNase III enzyme Drosha at serine 300 or serine 302 is required for its nuclear localization (2010), Nucleic Acids Res., 38, 6610-6619.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.26.3 expression of GFP-tagged wild-type and mutant enzymes in nuclei of HeLa, HEK293T, and Huh-7 cells Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
3.1.26.3 S300A site-directed mutagenesis, the mutant localizes to the nucleus like the wild-type enzyme Drosophila melanogaster
3.1.26.3 S300A/S302A site-directed mutagenesis, the double mutation completely disrupts nuclear localization Drosophila melanogaster
3.1.26.3 S300E site-directed mutagenesis, the mutant localizes to the nucleus like the wild-type enzyme Drosophila melanogaster
3.1.26.3 S300E/S302D site-directed mutagenesis, the mutant localizes to the nucleus like the wild-type enzyme Drosophila melanogaster
3.1.26.3 S302A site-directed mutagenesis, the mutant localizes to the nucleus like the wild-type enzyme Drosophila melanogaster
3.1.26.3 S302D site-directed mutagenesis, the mutant localizes to the nucleus like the wild-type enzyme Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.26.3 nucleus phosphorylation at Ser300 or Ser302 locates Drosha to the nucleus. Nuclear localization of Drosha is critical for its functionality in miRNA processing Drosophila melanogaster 5634
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.3 Drosophila melanogaster
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.26.3 phosphoprotein two phosphorylation sites at Ser300 and Ser302, which locates Drosha to the nucleus Drosophila melanogaster

Subunits

EC Number Subunits Comment Organism
3.1.26.3 More the RNA binding and enzymatic domains of Drosha are located on its C-terminus, the N-terminus harbors a nuclear localization signal Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
3.1.26.3 Drosha
-
 Drosophila melanogaster
3.1.26.3 RNase III
-
 Drosophila melanogaster

General Information

EC Number General Information Comment Organism
3.1.26.3 additional information the RNA binding and enzymatic domains of Drosha are located on its C-terminus, the N-terminus harbors a nuclear localization signal Drosophila melanogaster
3.1.26.3 physiological function the RNaseIII enzyme Drosha plays a pivotal role in microRNA, miRNA, biogenesis by cleaving primary miRNA transcripts to generate precursor miRNA in the nucleus. Nuclear localization of Drosha is critical for its functionality in miRNA processing Drosophila melanogaster