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Literature summary extracted from

  • Ishibashi, T.; Hayakawa, H.; Ito, R.; Miyazawa, M.; Yamagata, Y.; Sekiguchi, M.
    Mammalian enzymes for preventing transcriptional errors caused by oxidative damage (2005), Nucleic Acids Res., 33, 3779-3784.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.56 the increase in the production of erroneous proteins by oxidative damage is 28fold over the wild-type cells in Escherichia coli mutT deficient cells. By the expression of MTH1 in the cells, it is reduced to 1.2fold Homo sapiens
3.6.1.58 the increase in the production of erroneous proteins by oxidative damage is 28fold over the wild-type cells in Escherichia coli mutT deficient cells. By the expression of NUDT5 in the cells, it is reduced to 1.4fold Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.56 0.044
-
 8-oxo-GDP pH 8.0, 30°C Homo sapiens
3.6.1.56 0.29
-
 8-oxo-GTP pH 8.0, 30°C Homo sapiens
3.6.1.56 0.79
-
 GTP pH 8.0, 30°C Homo sapiens
3.6.1.58 0.0049
-
 8-oxo-GDP pH 8.0, 30°C Homo sapiens
3.6.1.58 0.048
-
 GDP pH 8.0, 30°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.55 8-oxo-GTP + H2O Escherichia coli the MutT protein eliminates 8-oxoGTP and prevents the occurrence of transcriptional errors, which are induced particularly in the aerobic state 8-oxo-GMP + diphosphate
-
 ?
3.6.1.56 8-oxo-GTP + H2O Homo sapiens this enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP 8-oxo-GMP + diphosphate
-
 ?
3.6.1.58 8-oxo-GDP + H2O Homo sapiens
-
 8-oxo-GMP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.55 Escherichia coli
-
-
-
3.6.1.56 Homo sapiens
-
-
-
3.6.1.58 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.55 8-oxo-dGTP + H2O
-
 Escherichia coli 8-oxo-dGMP + diphosphate
-
 ?
3.6.1.55 8-oxo-GTP + H2O the MutT protein eliminates 8-oxoGTP and prevents the occurrence of transcriptional errors, which are induced particularly in the aerobic state Escherichia coli 8-oxo-GMP + diphosphate
-
 ?
3.6.1.56 8-oxo-GDP + H2O Vmax/Km is 4% of the value for 8-oxo-GTP Homo sapiens 8-oxo-GMP + phosphate
-
 ?
3.6.1.56 8-oxo-GTP + H2O this enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP Homo sapiens 8-oxo-GMP + diphosphate
-
 ?
3.6.1.56 8-oxo-GTP + H2O the MTH1 protein has the ability to cleave the phosphoanhydride bond between the alpha and the beta phosphate of 8-oxoguanine-containing nucleoside di- and triphosphates. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP Homo sapiens 8-oxo-GMP + diphosphate
-
 ?
3.6.1.56 GTP + H2O Vmax/Km is 31% of the value for 8-oxo-GTP Homo sapiens GMP + diphosphate
-
 ?
3.6.1.56 additional information Vmax/Km for GDP is 0.3% of the value for 8-oxo-GTP Homo sapiens ?
-
 ?
3.6.1.58 8-oxo-GDP + H2O
-
 Homo sapiens 8-oxo-GMP + phosphate
-
 ?
3.6.1.58 8-oxo-GDP + H2O 8-oxoGDP is a specific substrate for the human NUDT5 protein Homo sapiens 8-oxo-GMP + phosphate
-
 ?
3.6.1.58 GDP + H2O Vmax/Km is 6.5% of the value for 8-oxo-GDP Homo sapiens GMP + phosphate
-
 ?
3.6.1.58 additional information NUDT5 does not degrade 8-oxo-GTP to the monophosphate (Vmax/Km is 0.7% of the activity with 8-oxo-GDP) Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.55 MutT
-
 Escherichia coli
3.6.1.56 MTH1
-
 Homo sapiens
3.6.1.58 NUDT5
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.56 30
-
 assay at Homo sapiens
3.6.1.58 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.56 8
-
 assay at Homo sapiens
3.6.1.58 8
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
3.6.1.55 malfunction the increase in the production of erroneous proteins by oxidative damage is 28fold over the wild-type cells in Escherichia coli mutT deficient cells Escherichia coli
3.6.1.55 physiological function is produced in cells by reactive oxygen species normally formed during cellular metabolic processes. This oxidized base can pair with both adenine and cytosine, and thus the existence of this base in messenger RNA would cause translational errors. The MutT protein of Escherichia coli degrades 8-oxoGua-containing ribonucleoside di- and triphosphates to the monophosphate, thereby preventing the misincorporation of 8-oxoGua into RNA Escherichia coli
3.6.1.56 physiological function is produced in cells by reactive oxygen species normally formed during cellular metabolic processes. This oxidized base can pair with both adenine and cytosine, and thus the existence of this base in messenger RNA would cause translational errors. The elimination of 8-oxoguanine-containing ribonucleotides from the precursor pool is important to ensure accurate protein synthesis. Both NUDT5 and MTH1 are involved in this process in human cells Homo sapiens
3.6.1.58 physiological function is produced in cells by reactive oxygen species normally formed during cellular metabolic processes. This oxidized base can pair with both adenine and cytosine, and thus the existence of this base in messenger RNA would cause translational errors. The elimination of 8-oxoguanine-containing ribonucleotides from the precursor pool is important to ensure accurate protein synthesis. Both NUDT5 and MTH1 are involved in this process in human cells Homo sapiens