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Literature summary extracted from

  • Riegler, H.; Geserick, C.; Zrenner, R.
    Arabidopsis thaliana nucleosidase mutants provide new insights into nucleoside degradation (2011), New Phytol., 191, 349-359.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.1 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.2.1 cytosol
-
 Arabidopsis thaliana 5829
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.1 Arabidopsis thaliana
-
 ecotype Columbia-0
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.1 Ni-NTA agarose column chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.1 leaf
-
 Arabidopsis thaliana
-
3.2.2.1 root
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.1 adenosine + H2O
-
 Arabidopsis thaliana D-ribose + adenine
-
 ?
3.2.2.1 guanosine + H2O preferred substrate Arabidopsis thaliana D-ribose + guanine
-
 ?
3.2.2.1 inosine + H2O preferred substrate Arabidopsis thaliana D-ribose + hypoxanthine
-
 ?
3.2.2.1 uridine + H2O substrate for isoform URH1, but nor URH2 Arabidopsis thaliana D-ribose + uracil
-
 ?
3.2.2.1 xanthosine + H2O
-
 Arabidopsis thaliana D-ribose + xanthine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.1 purine hydrolase
-
 Arabidopsis thaliana
3.2.2.1 URH1 isoform Arabidopsis thaliana
3.2.2.1 URH2 isoform Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
3.2.2.1 physiological function both isoforms URH1 and URH2 are unimportant for seedling establishment and plant growth, both isoform URH1 and URH2 are required for efficient inosine and xanthosine hydrolytic activity Arabidopsis thaliana