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Literature summary extracted from

  • Jermsuntiea, W.; Aki, T.; Toyoura, R.; Iwashita, K.; Kawamoto, S.; Ono, K.
    Purification and characterization of intracellular lipase from the polyunsaturated fatty acid-producing fungus Mortierella alliacea (2011), New Biotechnol., 28, 158-164.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.23 synthesis the highly stable Mortierella alliacea lipase may be useful for the synthesis of structured lipids, particularly acylglycerols containing functional unsaturated fatty acids at the sn-2 position Mortierella alliacea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.23 2-mercaptoethanol 50% residual activity at 1 mM Mortierella alliacea
3.1.1.23 Co2+ 47% residual activity at 1 mM Mortierella alliacea
3.1.1.23 Cu2+ 35% residual activity at 1 mM Mortierella alliacea
3.1.1.23 diethyldicarbonate 20% residual activity at 1 mM Mortierella alliacea
3.1.1.23 diisopropylfluorophosphate 75% residual activity at 1 mM Mortierella alliacea
3.1.1.23 EDTA 50% residual activity at 1 mM Mortierella alliacea
3.1.1.23 Fe3+ 35% residual activity at 1 mM Mortierella alliacea
3.1.1.23 Hg2+ 30% residual activity at 1 mM Mortierella alliacea
3.1.1.23 Mn2+ 58% residual activity at 1 mM Mortierella alliacea
3.1.1.23 p-chloromercuribenzoic acid 45% residual activity at 1 mM Mortierella alliacea
3.1.1.23 phenylmethylsulfonyl fluoride 70% residual activity at 1 mM Mortierella alliacea
3.1.1.23 Zn2+ 27% residual activity at 1 mM Mortierella alliacea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.23 Ca2+ 129% activity at 1 mM Mortierella alliacea
3.1.1.23 additional information not activated by K+ Mortierella alliacea
3.1.1.23 Na+ 110% activity at 1 mM Mortierella alliacea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.23 10965
-
 x * 10965, MALDI-TOF mass spectrometry Mortierella alliacea
3.1.1.23 11000
-
 x * 11000, SDS-PAGE Mortierella alliacea

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.23 Mortierella alliacea
-
-
-
3.1.1.23 Mortierella alliacea YN-15
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.23 acetone precipitation, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration Mortierella alliacea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.23 29
-
 crude enzyme, using olive oil as substrate, at pH 7.4, 30°C Mortierella alliacea
3.1.1.23 179
-
 after 6.2fold purification, using olive oil as substrate, at pH 7.4, 30°C Mortierella alliacea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.23 1-monocaprylin + H2O
-
 Mortierella alliacea ?
-
 ?
3.1.1.23 1-monolinolein + H2O one of the most preferred substrates Mortierella alliacea ?
-
 ?
3.1.1.23 1-monolinolenin + H2O best substrate Mortierella alliacea ?
-
 ?
3.1.1.23 1-monoolein + H2O one of the most preferred substrates Mortierella alliacea ?
-
 ?
3.1.1.23 1-monopalmitin + H2O
-
 Mortierella alliacea ?
-
 ?
3.1.1.23 2-monolinolein + H2O
-
 Mortierella alliacea ?
-
 ?
3.1.1.23 2-monoolein + H2O
-
 Mortierella alliacea ?
-
 ?
3.1.1.23 methylcaprylate + H2O low activity Mortierella alliacea ?
-
 ?
3.1.1.23 methyloleate + H2O low activity Mortierella alliacea ?
-
 ?
3.1.1.23 additional information monoacylglycerols containing long-chain unsaturated fatty acids and phosphatidylcholine are preferentially hydrolyzed over triacylglycerols and fatty acid methyl esters. Triacylglycerol may be formed via 2-monoacylglycerol Mortierella alliacea ?
-
 ?
3.1.1.23 additional information the enzyme also uses soybean oil, sesame oil, rapeseed oil, camellia oil, olive oil and linseed oil as substrates. 1,3-dicaprylin, 1,3-dipalmitin, 1,2-diolein, 1,3-diolein, 1,2-dilinolein, methylbutyrate, methyllaurate, methylpalmitate, methylpamitoleate, methylsterate, methyllinoleate, methyllinolenate, and methyleicosapentaenoate yield no detectable activity at either 30 or 50°C Mortierella alliacea ?
-
 ?
3.1.1.23 additional information monoacylglycerols containing long-chain unsaturated fatty acids and phosphatidylcholine are preferentially hydrolyzed over triacylglycerols and fatty acid methyl esters. Triacylglycerol may be formed via 2-monoacylglycerol Mortierella alliacea YN-15 ?
-
 ?
3.1.1.23 additional information the enzyme also uses soybean oil, sesame oil, rapeseed oil, camellia oil, olive oil and linseed oil as substrates. 1,3-dicaprylin, 1,3-dipalmitin, 1,2-diolein, 1,3-diolein, 1,2-dilinolein, methylbutyrate, methyllaurate, methylpalmitate, methylpamitoleate, methylsterate, methyllinoleate, methyllinolenate, and methyleicosapentaenoate yield no detectable activity at either 30 or 50°C Mortierella alliacea YN-15 ?
-
 ?
3.1.1.23 phosphatidylcholine + H2O one of the most preferred substrates Mortierella alliacea ?
-
 ?
3.1.1.23 phosphatidylcholine + H2O one of the most preferred substrates Mortierella alliacea YN-15 ?
-
 ?
3.1.1.23 tricaprylin + H2O
-
 Mortierella alliacea ?
-
 ?
3.1.1.23 tricaprylin + H2O
-
 Mortierella alliacea YN-15 ?
-
 ?
3.1.1.23 trieicosapentaenoin + H2O low activity Mortierella alliacea ?
-
 ?
3.1.1.23 trilaurin + H2O low activity Mortierella alliacea ?
-
 ?
3.1.1.23 trilinolein + H2O low activity Mortierella alliacea ?
-
 ?
3.1.1.23 trilinolenin + H2O low activity Mortierella alliacea ?
-
 ?
3.1.1.23 triolein + H2O the lipase strongly hydrolyzes the sn-1/3 ester bonds and weakly hydrolyzes the sn-2 ester bonds of triolein Mortierella alliacea ?
-
 ?
3.1.1.23 triolein + H2O the lipase strongly hydrolyzes the sn-1/3 ester bonds and weakly hydrolyzes the sn-2 ester bonds of triolein Mortierella alliacea YN-15 ?
-
 ?
3.1.1.23 tripalmitin + H2O
-
 Mortierella alliacea ?
-
 ?
3.1.1.23 tripalmitolein + H2O
-
 Mortierella alliacea ?
-
 ?
3.1.1.23 tristearin + H2O
-
 Mortierella alliacea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.23 ? x * 11000, SDS-PAGE Mortierella alliacea
3.1.1.23 ? x * 10965, MALDI-TOF mass spectrometry Mortierella alliacea

Synonyms

EC Number Synonyms Comment Organism
3.1.1.23 lipase
-
 Mortierella alliacea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.23 50
-
-
 Mortierella alliacea

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.23 20 80
-
 Mortierella alliacea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.23 70
-
 the enzyme is completely inactivated when preincubated for 1 h at over 70°C Mortierella alliacea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.23 9
-
-
 Mortierella alliacea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.23 2 12
-
 Mortierella alliacea

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.1.23 1
-
 in 50 mM phosphate buffer (pH 7.4), at 30°C Mortierella alliacea EDTA
3.1.1.23 1
-
 in 50 mM phosphate buffer (pH 7.4), at 30°C Mortierella alliacea 2-mercaptoethanol