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Literature summary extracted from

  • Aas, P.A.; Otterlei, M.; Falnes, P.O.; Vagbo, C.B.; Skorpen, F.; Akbari, M.; Sundheim, O.; Bjoras, M.; Slupphaug, G.; Seeberg, E.; Krokan, H.E.
    Human and bacterial oxidative demethylases repair alkylation damage in both RNA and DNA (2003), Nature, 421, 859-863.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.33 expressed in Escherichia coli BL21(DE3) cells Escherichia coli
1.14.11.33 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.11.33 nucleus
-
 Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.33 Fe2+ required for activity Escherichia coli
1.14.11.33 Fe2+ required for activity Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.33 methylated double-stranded bacteriophage lambda + 2-oxoglutarate + O2 Homo sapiens
-
 double-stranded bacteriophage lambda + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 methylated RNA bacteriophage MS2 + 2-oxoglutarate + O2 Homo sapiens isoform ABH3 reactivates methylated single-stranded RNA bacteriophage MS2 RNA bacteriophage MS2 + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 additional information Homo sapiens isoform ABH2 does not reactivate methylated RNA bacteriophage MS2 or methylated double-stranded bacteriophage lambda ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.33 Escherichia coli
-
-
-
1.14.11.33 Homo sapiens
-
-
-
1.14.11.33 Homo sapiens Q96Q83 isoform ABH3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.33 Ni-NTA column chromatography and UnoS column chromatography Escherichia coli
1.14.11.33 Ni-NTA column chromatography and UnoS column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.33 DNA-1-methyladenine + 2-oxoglutarate + O2
-
 Escherichia coli DNA-adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-1-methyladenine + 2-oxoglutarate + O2
-
 Homo sapiens DNA-adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-3-methylcytosine + 2-oxoglutarate + O2
-
 Escherichia coli DNA-cytosine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-3-methylcytosine + 2-oxoglutarate + O2
-
 Homo sapiens DNA-cytosine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 methylated double-stranded bacteriophage lambda + 2-oxoglutarate + O2
-
 Homo sapiens double-stranded bacteriophage lambda + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 methylated RNA bacteriophage MS2 + 2-oxoglutarate + O2 isoform ABH3 reactivates methylated single-stranded RNA bacteriophage MS2 Homo sapiens RNA bacteriophage MS2 + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 additional information isoform ABH2 does not reactivate methylated RNA bacteriophage MS2 or methylated double-stranded bacteriophage lambda Homo sapiens ?
-
 ?
1.14.11.33 additional information ABH2 does not repair RNA. ABH2 acts more efficiently on double-stranded DNA Homo sapiens ?
-
 ?
1.14.11.33 additional information ABH3 also repairs RNA. ABH3 prefers single-stranded nucleic acids Homo sapiens ?
-
 ?
1.14.11.33 additional information AlkB also repairs RNA. AlkB prefers single-stranded nucleic acids. Escherichia coli ?
-
 ?
1.14.11.33 additional information repair of alkylated poly(A) by ABH3 is almost as efficient as repair of poly(dA), and repair of poly(C) is substantially more efficient than repair of poly(dA), although not as good as repair of poly(dC) and single-stranded bacteriophage M13 DNA Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.33 ABH2 isoform Homo sapiens
1.14.11.33 ABH3 isoform Homo sapiens
1.14.11.33 AlkB
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.33 2-oxoglutarate
-
 Escherichia coli
1.14.11.33 2-oxoglutarate
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
1.14.11.33 physiological function isoform ABH2 repairs DNA close to the replication forks, is more active on double-stranded than on single-stranded DNA and is present in different subnuclear compartments at different stages of the cell cycle Homo sapiens
1.14.11.33 physiological function the nuclear localization of ABH3 and the clear preference for single-stranded DNA and RNA suggest that ABH3 has a role in maintenance of nuclear single-stranded DNA and RNA, with genes undergoing transcription potentially being its main targets Homo sapiens